Laboratory of Treatment and Valorization of Water Rejects (LTVRH), Water Researches and Technologies Center (CERTE), University of Carthage, Nabeul, Tunisia.
Department of Agriculture, Forest and Food Sciences, University of Torino, Torino, Italy.
Water Environ Res. 2020 Dec;92(12):2041-2048. doi: 10.1002/wer.1363. Epub 2020 Jun 19.
The wastewater from the dumping site usually contains high pollutant levels. Biological process and physico-chemical treatments are among several technologies for wastewater treatment. Using microorganisms in the treatment of landfill leachate is an emerging research issue. Furthermore, bioremediation is a feasible approach for pollutants removal from landfill leachate which would provide an efficient way to resolve the issue of landfill leachate. In this study, the performance of yeast and bacteria isolated from kefir grains was assessed for landfill leachate treatment. Kefir grains microbial composition was evaluated by molecular approaches (Rep-PCR and 16S rRNA gene sequencing). The obtained outcomes denoted that high concentrations of lactic acid bacteria and yeast populations (over 10 CFU/ml) were found in the kefir grains and were essentially composed of Lactococcus lactis, Lactobaccillus kefirien, bacillus sp., L. lactis, and Kluyveromyces marxianus. The co-culture with 1% of inoculum size was demonstrated as the most efficient in the degradation of different contaminants. The overall abatement rate of chemical oxygen demand (COD), ammonium nitrogen ( ), and salinity were 75.8%, 85.9%, and 75.13%, respectively. The bioremediation process resulted in up of 75% removal efficiency of Ni and Cd, and a 73.45%, 68.53%, and a 58.17% removal rates of Cu, Pb, and Fe, respectively. The research findings indicate the performance of L. lactis and K. marxianus co-culture isolated from kefir grains for the bioremediation of LFL. PRACTITIONER POINTS: Isolation and identification of microorganisms from kefir grains was carried out. Biological treatment of LFL using monoculture of (Lactoccocus lactis; Kluyveromyces marxianus) and co-culture (5% of L. lactis and 5% K. marxianus) has been performed. Biological treatment using co-culture strain is an effective approach to remove organic matter, and heavy metals.
垃圾填埋场的废水通常含有较高的污染物水平。生物处理和物理化学处理是废水处理的几种技术之一。利用微生物处理垃圾渗滤液是一个新兴的研究课题。此外，生物修复是去除垃圾渗滤液中污染物的一种可行方法，它为解决垃圾渗滤液问题提供了一种有效的方法。在本研究中，评估了从克菲尔粒中分离出的酵母和细菌对垃圾渗滤液处理的性能。通过分子方法（Rep-PCR 和 16S rRNA 基因测序）评估了克菲尔粒微生物的组成。研究结果表明，克菲尔粒中存在高浓度的乳酸菌和酵母种群（超过 10 个 CFU/ml），主要由乳球菌、发酵乳杆菌、芽孢杆菌、乳杆菌和马克斯克鲁维酵母组成。当接种量为 1%时，共培养被证明是降解不同污染物最有效的方法。化学需氧量（COD）、铵氮（ ）和盐度的总体去除率分别为 75.8%、85.9%和 75.13%。生物修复过程导致 Ni 和 Cd 的去除效率提高了 75%，Cu、Pb 和 Fe 的去除率分别提高了 73.45%、68.53%和 58.17%。研究结果表明，从克菲尔粒中分离出的乳杆菌和马克斯克鲁维酵母共培养物对 LFL 的生物修复具有良好的效果。
从克菲尔粒中进行了微生物的分离和鉴定。
使用（乳球菌；马克斯克鲁维酵母）的单培养物和共培养物（5%的乳杆菌和 5%的马克斯克鲁维酵母）对 LFL 进行了生物处理。
共培养菌株的生物处理是去除有机物和重金属的有效方法。
