Xinjiang Institute of Traditional Uygur Medicine, Urumqi, Xinjiang, 830011, China.
Xinjiang Production and Construction Corps Center Blood Station, Urumqi, Xinjiang, 830000, China.
J Food Sci. 2020 Jun;85(6):1897-1906. doi: 10.1111/1750-3841.15149. Epub 2020 May 25.
The anshenyizhi compound (AC), a mixture from Chinese medicine herbs, has numerous biological effects. In the present study, the acute exercise-treated mice model was established to explore the antifatigue properties of AC and its underlying mechanisms. AC increased exercise endurance in the weight-loaded forced swimming test and rota-rod test. The antifatigue properties of AC were closely correlated with enhancing the body's exercise endurance by increasing the levels of cyanmethemoglobin, testosterone/corticosterone, and creatine kinase, while decreasing the levels of lactic acid, lactate dehydrogenase, and blood urea nitrogen in serum. Moreover, our results confirmed the antioxidant ability of AC by improving the activities of superoxide dismutase while reducing reactive oxygen species and malondialdehyde levels in serum. The AC also improved the storage of glycogen by increasing the levels of succinate dehydrogenase, and malate dehydrogenase in liver and muscle. Additionally, AC displayed the antifatigue and antiapoptosis effects via regulating Nrf2-mediated oxidative stress, AMPK-related glucose metabolism, and p53 pathways. Our experimental results first provided a support that AC had effects on antifatigue through regulating AMPK/PGC-1α-related energy metabolism and Nrf2/ARE-mediated oxidative stress. Consequently, AC could be developed into a new functional food supplement for the prevention and treatment of diseases related to fatigue in the future.
安神益智复方（AC）是一种中药混合物，具有多种生物学效应。本研究建立了急性运动处理的小鼠模型，以探讨 AC 的抗疲劳特性及其作用机制。AC 增加了负重力竭游泳试验和转棒试验中小鼠的运动耐力。AC 的抗疲劳特性与通过增加氰高铁血红蛋白、睾酮/皮质酮和肌酸激酶的水平，同时降低血清中乳酸、乳酸脱氢酶和血尿素氮的水平，从而增强机体的运动耐力密切相关。此外，我们的结果通过提高超氧化物歧化酶的活性，同时降低血清中活性氧和丙二醛的水平，证实了 AC 的抗氧化能力。AC 还通过增加肝脏和肌肉中琥珀酸脱氢酶和苹果酸脱氢酶的水平来改善糖原的储存。此外，AC 通过调节 Nrf2 介导的氧化应激、AMPK 相关的葡萄糖代谢和 p53 途径，显示出抗疲劳和抗细胞凋亡的作用。我们的实验结果首次提供了支持，即 AC 通过调节 AMPK/PGC-1α 相关的能量代谢和 Nrf2/ARE 介导的氧化应激对抗疲劳有作用。因此，AC 未来可能被开发成一种新的功能性食品补充剂，用于预防和治疗与疲劳相关的疾病。
