Department of Physiology, Immunology Laboratory, University of Calcutta, University Colleges of Science and Technology, Calcutta, West Bengal, India.
Department of Physiology, Immunology Laboratory, University of Calcutta, University Colleges of Science and Technology, Calcutta, West Bengal, India.
J Neuroimmunol. 2020 Jul 15;344:577262. doi: 10.1016/j.jneuroim.2020.577262. Epub 2020 May 12.
Microglial inflammation plays a pivotal role in the pathogenesis of S. aureus induced brain abscesses. The objective of this study was to regulate microglial activation by the combinatorial treatment of ciprofloxacin either with dexamethasone or celecoxib via targeting M1 and M2 polarization. The antibiotic-immunomodulator combinations were applied either by opening both TLR-2 and GR or neutralizing each of them. Our results confirmed that dexamethasone along with ciprofloxacin attenuated bacterial burden along with ROS production more efficiently than celecoxib combination during TLR-2 neutralization. FACS data indicated microglial M1 to M2 switching that was responsible for the better resolution of microglial inflammation.
小胶质细胞炎症在金黄色葡萄球菌诱导的脑脓肿发病机制中起着关键作用。本研究的目的是通过联合应用环丙沙星与地塞米松或塞来昔布来调节小胶质细胞的激活，从而靶向 M1 和 M2 极化。通过同时打开 TLR-2 和 GR 或分别中和它们来应用抗生素-免疫调节剂组合。我们的结果证实，在 TLR-2 中和期间，地塞米松与环丙沙星联合使用比塞来昔布联合使用更有效地减轻细菌负担和 ROS 产生。FACS 数据表明小胶质细胞 M1 向 M2 的转换是小胶质细胞炎症更好缓解的原因。
