Barber K G, Kitts D D, Townsley P M, Smith D S
Department of Food Science, Faculty of Agricultural Sciences, University of British Columbia, Canada.
Toxicon. 1988;26(11):1027-34. doi: 10.1016/0041-0101(88)90201-2.
This paper provides evidence for a protein component which appears to be involved in the seasonal resistance of small shore crabs, Hemigrapsus oregonesis and Hemigrapsus nudus to saxitoxin, a principle neurotoxin involved in paralytic shellfish poisoning (PSP). This unique protein complex was isolated and partially purified by ion exchange chromatography using DEAE-cellulose from visceral tissue extracts of resistant crabs. The complex was absent in control crabs that were sensitive to saxitoxin. In addition, the protein complex was induced in the crab after acute administration of low doses of saxitoxin. Results indicate that the protein complex is acidic in nature and has an apparent mol. wt of 145,000.
本文提供了证据，证明存在一种蛋白质成分，它似乎与小型岸蟹（俄勒冈拟滨蟹和光背拟滨蟹）对石房蛤毒素的季节性抗性有关，石房蛤毒素是一种导致麻痹性贝类中毒（PSP）的主要神经毒素。使用DEAE-纤维素通过离子交换色谱法从抗性蟹的内脏组织提取物中分离并部分纯化了这种独特的蛋白质复合物。对石房蛤毒素敏感的对照蟹中不存在该复合物。此外，在低剂量石房蛤毒素急性给药后，蟹体内会诱导产生该蛋白质复合物。结果表明，该蛋白质复合物本质上呈酸性，表观分子量为145,000。
