Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina, Londrina, PR, 86057-970, Brazil.
Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, Brazil.
Environ Sci Pollut Res Int. 2020 Aug;27(24):30222-30231. doi: 10.1007/s11356-020-09315-w. Epub 2020 May 25.
Surfactants represent a billionaire market of amphiphilic molecules with worldwide applications in almost every branch of modern industry. The most common surfactants, available and currently used, are chemically produced. However, there is an urge to replace these chemical compounds with those obtained by mild and green technologies such as microbial biosurfactants produced by fermentative processes. Rhamnolipids are glycolipid biosurfactants that present highly effective surface-active properties and enormous market potential; nevertheless, their production costs remain not competitive. Here, we present a process of rhamnolipid production by static submerged cultivation using membranes of bacterial cellulose as substrate. The mixture of the rhamnolipid congeners was characterized showing effective surface-active properties and high amount of di-rhamnolipids (95.6%). Through this fermentative technology, 15.8 g/L of rhamnolipid was reach using a very simple and low-cost medium. The present process might decrease biosurfactant production cost, avoid foam formation, and finally make rhamnolipid production more viable.
表面活性剂是一种价值数十亿美元的两亲分子市场，在现代工业的几乎每一个分支中都有全球应用。最常见的表面活性剂是化学合成的，目前也在使用。然而，人们迫切希望用温和、绿色的技术来取代这些化学化合物，比如通过发酵工艺生产的微生物生物表面活性剂。鼠李糖脂是糖脂生物表面活性剂，具有非常高的表面活性和巨大的市场潜力；然而，其生产成本仍然没有竞争力。在这里，我们介绍了一种使用细菌纤维素膜作为基质进行静态浸没培养生产鼠李糖脂的方法。对混合的鼠李糖脂同系物进行了表征，结果表明其具有有效的表面活性和高含量的二鼠李糖脂（95.6%）。通过这种发酵技术，使用非常简单和低成本的培养基可达到 15.8 g/L 的鼠李糖脂产量。本工艺可以降低生物表面活性剂的生产成本，避免泡沫形成，最终使鼠李糖脂的生产更具可行性。
