VIDO-InterVac, University of Saskatchewan, SK, Canada; Veterinary Microbiology, WCVM, University of Saskatchewan, Saskatoon, SK, Canada.
VIDO-InterVac, University of Saskatchewan, SK, Canada; Shandong Binzhou Animal Science & Veterinary Medicine Academy, 256600, Binzhou, Shandong, China.
Virology. 2020 Jul;546:25-37. doi: 10.1016/j.virol.2020.04.006. Epub 2020 Apr 11.
Bovine adenovirus-3 (BAdV-3) is a non enveloped, icosahedral DNA virus containing a genome of 34446 bps. The intermediate region of BAdV-3 encodes pIX and IVa2 proteins. Here, we report the characterization of BAdV-3 IVa2. Anti-IVa2 serum detected a 50 kDa protein at 24-48 h post infection in BAdV-3 infected cells. The IVa2 localizes to nucleus and nucleolus of BAdV-3 infected cells. Analysis of mutant IVa2 demonstrated that amino acids 1-25 and 373-448 are required for nuclear and nucleolar localization of IVa2, respectively. The nuclear import of IVa2 utilize importin α -1 of importin nuclear import pathway. Although deletion/substitution of amino acids 4-18 is sufficient to abrogate the nuclear localization of IVa2, amino acids 1-25 are required for nuclear localization of a cytoplasmic protein. Furthermore, we demonstrate that amino acids 1-25 and 120-140 of IVa2 interact with importin α-1 and pV proteins, respectively in BAdV-3 infected cells.
牛腺病毒 3 型（Bovine adenovirus-3，BAdV-3）是一种无包膜的二十面体 DNA 病毒，基因组大小为 34446 个碱基对。BAdV-3 的中间区编码 pIX 和 IVa2 蛋白。在此，我们报告了 BAdV-3 IVa2 的特征。抗-IVa2 血清在 BAdV-3 感染的细胞中于感染后 24-48 小时检测到 50 kDa 的蛋白。IVa2 定位于 BAdV-3 感染细胞的核和核仁中。对突变体 IVa2 的分析表明，氨基酸 1-25 和 373-448 分别是 IVa2 核定位和核仁定位所必需的。IVa2 的核输入利用了核输入途径中的 importin α-1。尽管氨基酸 4-18 的缺失/取代足以阻断 IVa2 的核定位，但氨基酸 1-25 是细胞质蛋白核定位所必需的。此外，我们证明了在 BAdV-3 感染的细胞中，IVa2 的氨基酸 1-25 和 120-140 分别与 importin α-1 和 pV 蛋白相互作用。
