Ayaz Fatma, Küçükboyaci Nurgün, Bani Barış, Şener Bilge, Choudhary Muhammad Iqbal
Selçuk University, Faculty of Pharmacy, Department of Pharmacognosy, Konya, Turkey.
Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey.
Turk J Pharm Sci. 2018 Dec;15(3):382-385. doi: 10.4274/tjps.88700. Epub 2018 Nov 20.
The aim of this study was to investigate the probable toxicity on brine shrimp, phytotoxicity, and insecticidal activity of Aytac & Anderb.
The MeOH (80%) extract obtained from the whole plant of was fractionated through subsequent solvent extractions in increasing polarity with -hexane, chloroform, and -butanol. The MeOH (80%) extract and all fractions of were evaluated for their biologic activities using screening bioassays such as brine shrimp lethality test and phytotoxicity against , as well as insecticidal activity against and .
The findings showed that the -hexane and chloroform fractions of the plant had significant phytotoxic activities with 100% growth inhibition (GI) at 1000 μg/mL against . Moreover, the MeOH (80%) extract (53% GI) and -butanol fraction (46.6% GI) of the plant had moderate phytotoxic activities at 1000 μg/mL. Otherwise, no samples had toxicity on the brine shrimps. In addition, the remaining water fraction had low insecticidal activity with 20% mortality against .
Our results show that the n-hexane and chloroform fractions of had potential phytotoxic effects.
本研究旨在调查Aytac & Anderb对卤虫的潜在毒性、植物毒性及杀虫活性。
从全株植物中获得的甲醇(80%)提取物,通过依次用正己烷、氯仿和正丁醇进行极性递增的溶剂萃取进行分离。使用卤虫致死试验和对[具体植物名称]的植物毒性以及对[具体昆虫名称1]和[具体昆虫名称2]的杀虫活性等筛选生物测定法,对甲醇(80%)提取物及该植物的所有馏分的生物活性进行评估。
研究结果表明,该植物的正己烷和氯仿馏分具有显著的植物毒性活性,在1000μg/mL时对[具体植物名称]的生长抑制率(GI)达100%。此外该植物的甲醇(80%)提取物(GI为53%)和正丁醇馏分(GI为46.6%)在1000μg/mL时具有中等植物毒性活性。否则,没有样品对卤虫有毒性。此外,剩余的水相馏分对[具体昆虫名称]的杀虫活性较低,死亡率为20%。
我们的结果表明,[植物名称]的正己烷和氯仿馏分具有潜在的植物毒性作用。