Chen Chao-Ju, Hsieh Li-Ling, Lin Shu-Kai, Wang Chu-Feng, Huang Yi-Hui, Lin Shang-Yi, Lu Po-Liang
Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, Taiwan.
Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan.
Diagnostics (Basel). 2020 May 21;10(5):333. doi: 10.3390/diagnostics10050333.
Coronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly established methods in the novel viral pandemic and was considered as the gold standard for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this report, we illustrate our experience of applying a protocol from the Taiwan CDC and achieving assay optimization in the immediate circumstances to meet the urgent medical and public health needs.
2019冠状病毒病（COVID-19），即目前仍未得到控制的传染病疫情，已在全球范围内引发了重大挑战。全球都需要一种可靠的COVID-19快速诊断检测方法。实时逆转录聚合酶链反应是新型病毒大流行中建立最快的方法之一，被视为检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的金标准。在本报告中，我们阐述了应用台湾疾病控制中心方案的经验，并在当下实现了检测优化，以满足紧急医疗和公共卫生需求。
