Tao Xuefang, Wu Xiaotang, Huang Tao, Mu Deguang
Affiliated Hospital of Shaoxing University, Shaoxing, China.
Shanghai Engineering Research Center of Pharmaceutical Translation, Shanghai, China.
Front Genet. 2020 May 8;11:352. doi: 10.3389/fgene.2020.00352. eCollection 2020.
Lung cancer, the most common of malignant tumors, is typically of the non-small cell (NSCLC) type. T-cell-based immunotherapies are a promising and powerful approach to treating NSCLCs. To characterize the CD8 T cells of non-small cell lung cancer, we re-analyzed the published RNA-Seq gene expression profiles of 36 CD8 T cell isolated from tumor (TIL) samples and 32 adjacent uninvolved lung (NTIL) samples. With an advanced Monte Carlo method of feature selection, we identified the CD8 TIL specific expression patterns. These patterns revealed the key dysfunctional genes and pathways in CD8 TIL and shed light on the molecular mechanisms of immunity and use of immunotherapy.
肺癌是最常见的恶性肿瘤，通常为非小细胞肺癌（NSCLC）类型。基于T细胞的免疫疗法是治疗非小细胞肺癌的一种有前景且强大的方法。为了表征非小细胞肺癌的CD8 T细胞，我们重新分析了从肿瘤（TIL）样本中分离出的36个CD8 T细胞和32个相邻未受累肺组织（NTIL）样本已发表的RNA测序基因表达谱。通过先进的蒙特卡罗特征选择方法，我们确定了CD8 TIL特异性表达模式。这些模式揭示了CD8 TIL中的关键功能失调基因和通路，并阐明了免疫的分子机制以及免疫疗法的应用。
