Dong S R, Jiang J, Wang Y J, Li C L, Shi Y, Yang Y, Yang Y, Li L H, Cai B, You J B, Jiang F, Jiang Q W, Zhou Y B
Department of Epidemiology, School of Public Health, Fudan University, Key Laboratory of Public Health Safety, Ministry of Education, Tropical Disease Research Center, Fudan University, Shanghai 200032, China.
Junshan District Station for Schistosomiasis Control, Yueyang City, Hunan Province, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2020 Apr 8;32(2):132-139. doi: 10.16250/j.32.1374.2019202.
To evaluate the effects of water body environments on the microbial community of snails in marshlands of the eastern Dongting Lake where natural extinction of snails are found, so as to explore the correlation between the natural extinction of snails and the microbial community in snails.
Snails were caged water bodies in the Qianliang Lake marshland (Qianliang Lake regions) where natural extinction of snails was found and in the Junshan Park marshland (Junshan Park regions) in the eastern Dongting Lake for 30 days, and then all snails were collected and identified for survival or death. DNA sequencing of the fungi and bacteria was performed in snails before and after immersion in waters, and the biodiversity and abundance were analyzed.
The survival rates of snails were 28.0% (70/250) and 64.8% (162/250) in Qianliang Lake regions and Junshan Park regions 30 days after immersion in waters, respectively ( = 81.365, < 0.01). The number of the fungal community and the biodiversity of the bacterial community were both greater in snails caged in Qianliang Lake regions post-immersion than pre-immersion, and there was a significant difference in the structure of the fungal and bacterial communities. The microbial community with a significant difference included Flavobacteriaceae,which was harmful to snails.
The water body environment affects the composition of the microbial community in snails in marshlands with natural snail distinction around the eastern Dongting Lake; however, further studies are required to investigate whether the natural distinction of snails is caused by water body environments-induced changes of the microbial spectrum in snails.
评估水体环境对东洞庭湖湿地有钉螺自然消亡现象的滩地钉螺微生物群落的影响，以探讨钉螺自然消亡与钉螺体内微生物群落之间的相关性。
将钉螺置于发现有钉螺自然消亡现象的钱粮湖湿地（钱粮湖区域）及东洞庭湖君山公园湿地（君山公园区域）的水体中笼养30天，之后收集所有钉螺并鉴定其存活或死亡情况。对浸水处理前后钉螺体内的真菌和细菌进行DNA测序，并分析其生物多样性和丰度。
浸水处理30天后，钱粮湖区域和君山公园区域钉螺的存活率分别为28.0%（70/250）和64.8%（162/250）（χ² = 81.365，P < 0.01）。浸水处理后，钱粮湖区域笼养钉螺体内的真菌群落数量及细菌群落生物多样性均高于浸水处理前，且真菌和细菌群落结构存在显著差异。差异显著的微生物群落包括对钉螺有害的黄杆菌科。
水体环境影响东洞庭湖周边有钉螺自然消亡现象的湿地中钉螺体内微生物群落的组成；然而，钉螺的自然消亡是否由水体环境引起的钉螺体内微生物谱变化所致，仍需进一步研究。
