Tajuddin N, Druse M J
Department of Biochemistry and Biophysics, Loyola University Medical Center, Maywood, IL 60153.
Alcohol. 1988 Nov-Dec;5(6):461-4. doi: 10.1016/0741-8329(88)90083-3.
Previous studies by this laboratory found that in utero ethanol exposure results in a decrease of several presynaptic components of serotonin (5-HT) containing neurons in the cerebral cortex and cortical regions. The present study examined whether in utero ethanol exposure similarly affects the development of the cortical targets of serotonergic projections. Specifically, we examined the influence of in utero ethanol exposure on 5-HT2 binding sites in the motor and somatosensory cortices of the offspring of rats that consumed a control or 6.6% (v/v) ethanol liquid diet on a chronic basis prior to parturition. These studies demonstrated that neither the Kd nor Bmax for the binding of [3H]-ketanserin to membranes from the motor and somatosensory cortices of 19- and 35-day-old rats were significantly altered by in utero ethanol exposure. These results suggest that the development of the postsynaptic target areas of cortical serotonergic projections are more resistant to the effects of in utero ethanol exposure than the presynaptic components of these projections.
该实验室先前的研究发现，子宫内乙醇暴露会导致大脑皮层和皮质区域中含5-羟色胺（5-HT）神经元的几种突触前成分减少。本研究探讨子宫内乙醇暴露是否同样影响5-羟色胺能投射的皮质靶标的发育。具体而言，我们研究了子宫内乙醇暴露对大鼠后代运动和躯体感觉皮层中5-HT2结合位点的影响，这些大鼠在分娩前长期食用对照或6.6%（v/v）乙醇液体饲料。这些研究表明，子宫内乙醇暴露并未显著改变19日龄和35日龄大鼠运动和躯体感觉皮层膜上[3H]-酮舍林结合的解离常数（Kd）或最大结合量（Bmax）。这些结果表明，皮质5-羟色胺能投射的突触后靶区的发育比这些投射的突触前成分对子宫内乙醇暴露的影响更具抗性。
