Sarkar M, Mookerjea S
Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada.
Biochem Cell Biol. 1988 Dec;66(12):1265-9. doi: 10.1139/o88-146.
Inflammation and glucocorticoids stimulate hepatic glycoprotein synthesis, resulting in an increased secretion of serum glycoproteins. We now present evidence that the synthesis of dolichol and dolichol phosphate from mevalonate is increased in hepatocytes from inflamed rats. Also, in inflamed rats, the levels of dolichol and dolichol phosphate are increased in liver homogenates and microsomes. Dexamethasone treatment of the cells, however, does not increase the synthesis of dolichol and dolichol phosphate from mevalonate. The results suggest that the inflammation-induced dolichol-linked saccharide and glycoprotein synthesis is possibly mediated through an increase in the level of dolichol and dolichol phosphate in the liver. Since dexamethasone treatment does not increase the synthesis of dolichol and dolichol phosphate, its action on glycoprotein synthesis appears to be different and to affect the induction of enzymes in mannosyl phosphoryl dolichol- and dolichol-linked oligosaccharide synthesis.
炎症和糖皮质激素会刺激肝脏糖蛋白的合成，导致血清糖蛋白分泌增加。我们现在提供证据表明，在炎症大鼠的肝细胞中，由甲羟戊酸合成的多萜醇和磷酸多萜醇增加。此外，在炎症大鼠的肝脏匀浆和微粒体中，多萜醇和磷酸多萜醇的水平也升高。然而，用地塞米松处理细胞并不会增加由甲羟戊酸合成的多萜醇和磷酸多萜醇。结果表明，炎症诱导的多萜醇连接的糖类和糖蛋白合成可能是通过肝脏中多萜醇和磷酸多萜醇水平的增加介导的。由于地塞米松处理不会增加多萜醇和磷酸多萜醇的合成，其对糖蛋白合成的作用似乎不同，且会影响甘露糖基磷酸多萜醇和多萜醇连接的寡糖合成中酶的诱导。
