Kargaltseva N M, Kocherovets V I, Mironov A Y, Borisova O Y
G.N. Gabrichevskii Moscow research institute for epidemiology and microbiology for Rospotrebnadzor, 125212, Moscow, Russia.
I.M. Sechenov First Moscow State Medical University, 119991, Moscow, Russia.
Klin Lab Diagn. 2020;65(6):375-381. doi: 10.18821/0869-2084-2020-65-6-375-381.
When diagnosing bloodstream infection (BI) the culture medium is the basis for growth of microorganisms and obtaining the blood culture. Pancreatic digest from fish meal is the basis of all culture media in Russia. In European countries brain-heart media (BHM) are used for detecting microorganisms in blood. In Russia BHM is not produced. The aim is to work out the formulation and the way of the BHM (broth and agar) preparation in order to improve the efficiency of obtaining blood culture. There were defined the physical and chemical indices and biological parameters of the BHM. The microbiological control of the BHM was carried out by diagnostic study of cardiological patients' blood. On the basis of the developed technique of the brain-heart extraction (BHE) preparation there was created the liquid and agar BHM (LBHM, BHA). The LBHM was poured into bottles which then were filled with the inert gas. The bottles were closed with rubber stoppers and rolled in metal caps became a closed system. Microbiological qualities of LBHM were tested on 260 blood samples and thioglycollate medium (TGM) and LBHM. Aerobic microorganisms grew in LBHM 2,4 times more often than in TGM. The microaerophilic microbes grew in LBHM 3,2 times more often than in TGM. Anaerobic microbes did not show any growth in TGM, (p<0,001). Monomicrobes hemocultures were obtained in LBHM 13,4 times more often than in glucose broth and 2,3 times more often than in TGM, (p<0,001). Polymicrobes hemocultures were obtained in LBHM more often than in TGM (7,3% and 1,7%, respectively). The quality of brain-heart agar (BHA) was tested on 300 blood samples in 5% blood meat-pepton agar (MPA) and BHA in aerobic and anaerobic conditions for both the media. Aerobic microorganisms grew in BHA 2 times more often than in MPA. The microaerophilic microbes grew in BHA 3,6 times more often than in MPA. In anaerobic condition in BHA aerobic microorganisms grew 2 times more often than in MPA and the microaerophilic microbes grew 2,5 times more often than in MPA, (p<0,001). Anaerobic microbes did not grow in MPA. When diagnosing bloodstream infection the BHM (liquid and agar) are able to create the optimal conditions for the increase of the wide range pathogen growth.
诊断血流感染（BI）时，培养基是微生物生长及获取血培养物的基础。在俄罗斯，鱼粉胰酶消化物是所有培养基的基础成分。在欧洲国家，脑心培养基（BHM）用于检测血液中的微生物。俄罗斯不生产BHM。本研究旨在制定BHM（肉汤和琼脂）的配方及制备方法，以提高获取血培养物的效率。研究确定了BHM的理化指标和生物学参数。通过对心脏病患者血液的诊断性研究对BHM进行微生物学控制。基于所开发的脑心浸出物（BHE）制备技术，制备了液体和琼脂BHM（LBHM、BHA）。将LBHM倒入瓶中，然后充入惰性气体。瓶子用橡胶塞密封，再套上金属盖，形成一个封闭系统。在260份血样上对LBHM以及硫乙醇酸盐培养基（TGM）和LBHM的微生物学质量进行了检测。需氧微生物在LBHM中生长的频率比在TGM中高2.4倍。微需氧微生物在LBHM中生长的频率比在TGM中高3.2倍。厌氧微生物在TGM中未生长（p<0.001）。在LBHM中获得单微生物血培养物的频率比在葡萄糖肉汤中高13.4倍，比在TGM中高2.3倍（p<0.001）。在LBHM中获得多微生物血培养物的频率高于TGM（分别为7.3%和1.7%）。在5%血肉蛋白胨琼脂（MPA）和BHA中，在需氧和厌氧条件下，对300份血样检测了脑心琼脂（BHA）的质量。需氧微生物在BHA中生长的频率比在MPA中高2倍。微需氧微生物在BHA中生长的频率比在MPA中高3.6倍。在厌氧条件下，BHA中需氧微生物生长的频率比MPA中高2倍，微需氧微生物生长的频率比MPA中高2.5倍（p<0.001）。厌氧微生物在MPA中未生长。诊断血流感染时，BHM（液体和琼脂）能够为多种病原体的生长创造最佳条件。
