Boller K, Arpin M, Pringault E, Mangeat P, Reggio H
Laboratoire de Biologie, Différenciation Cellulaire, Faculté des Sciences de Marseille-Luminy, France.
Differentiation. 1988 Nov;39(1):51-7. doi: 10.1111/j.1432-0436.1988.tb00080.x.
The distribution of the mRNA encoding for villin, the major actin-binding protein of intestinal brush border, was studied during the differentiation of mouse intestinal epithelial cells and compared to the distribution of the protein. In situ hybridization using a cRNA clone specific for villin indicated that the distribution of the mRNA did not fully parallel that of the protein, although the overall labelling pattern for mRNA and protein along the crypt-villus axis was similar. While villin was present in equal amounts in all cells along the villi, villin-specific mRNA was mainly accumulated in the cells at the villus base, the area of the epithelium where terminal differentiation takes place and where the brush border is formed.
研究了小鼠肠上皮细胞分化过程中编码绒毛蛋白（肠刷状缘主要肌动蛋白结合蛋白）的mRNA分布，并将其与该蛋白的分布进行比较。使用绒毛蛋白特异性cRNA克隆进行原位杂交表明，尽管mRNA和蛋白沿隐窝-绒毛轴的整体标记模式相似，但mRNA的分布与蛋白的分布并不完全平行。虽然绒毛蛋白在绒毛上所有细胞中的含量相等，但绒毛蛋白特异性mRNA主要积聚在绒毛基部的细胞中，即上皮细胞发生终末分化并形成刷状缘的区域。
