Department of Molecular Chemistry, University Grenoble Alpes, CNRS, 38000 Grenoble, France.
Department of Chemical Sciences, University Federico II, 80126 Naples, Italy.
Int J Mol Sci. 2020 May 26;21(11):3741. doi: 10.3390/ijms21113741.
A chimeric enzyme based on the genetic fusion of a laccase with a hydrophobin domain was employed to functionalize few-layer graphene, previously exfoliated from graphite in the presence of the hydrophobin. The as-produced, biofunctionalized few-layer graphene was characterized by electrochemistry and Raman spectroscopy, and finally employed in the biosensing of phenols such as catechol and dopamine. This strategy paves the way for the functionalization of nanomaterials by hydrophobin domains of chimeric enzymes and their use in a variety of electrochemical applications.
一种基于漆酶与疏水蛋白结构域基因融合的嵌合酶被用于功能化少层石墨烯，这些少层石墨烯是先前在疏水蛋白存在的情况下从石墨中剥离得到的。所制备的生物功能化少层石墨烯通过电化学和拉曼光谱进行了表征，并最终用于检测儿茶酚和多巴胺等酚类物质的生物传感器。该策略为通过嵌合酶的疏水蛋白结构域对纳米材料进行功能化以及将其应用于各种电化学应用铺平了道路。
