Wang Mengyan, Jiang Ping, Yu Xiang, Mi Jiaqi, Bai Zitong, Zhang Xiuqi, Liu Yinuo, Fang Xibi, Yang Runjun, Zhao Zhihui
College of Agriculture, Guangdong Ocean University, Zhanjiang 524088, China.
College of Animal Science, Jilin University, Changchun 130062, China.
Animals (Basel). 2020 May 26;10(6):923. doi: 10.3390/ani10060923.
In this study, we precisely constructed and transfected the overexpression and interference vectors in BFFs to evaluate the role of gene on lipid metabolism in vitro. The expression of of DLK1 in the mRNA and protein level tended to reduce, and TGs were significantly increased in the pGPU6-shDLK1 group compared to the control group ( < 0.05). The expression of DLK1 in the mRNA and protein level were increased in the pBI-CMV3-DLK1 group compared to the control group, and the TGs content showed a significant decrease in the pBI-CMV3-DLK1 group ( < 0.05). Meanwhile, we used the restriction fragment length polymorphism (RFLP-PCR) detection method to screen SNPs further to explore and analyze the relationship between the gene and the economic traits of 28-month-old Chinese Simmental and the fatty acids composition of cattle longissimus muscle. The result showed that two SNPs, IVS3 + 478 C>T and IVS3 + 609 T>G, were identified as being significantly associated with carcass and meat quality traits in Chinese Simmental, such as the carcass fat coverage rate, loin eye muscle area, and fat color score. In summary, our results indicated that can affect lipid metabolism in bovine and these two SNPs might be applied as genetic markers of meat quality traits for beef cattle breeding.
在本研究中，我们精确构建并转染了牛胎儿成纤维细胞（BFFs）中的过表达和干扰载体，以在体外评估该基因对脂质代谢的作用。与对照组相比，pGPU6-shDLK1组中DLK1在mRNA和蛋白质水平的表达趋于降低，甘油三酯（TGs）显著升高（<0.05）。与对照组相比，pBI-CMV3-DLK1组中DLK1在mRNA和蛋白质水平的表达增加，并且pBI-CMV3-DLK1组中TGs含量显著降低（<0.05）。同时，我们使用限制性片段长度多态性（RFLP-PCR）检测方法进一步筛选单核苷酸多态性（SNPs），以探索和分析该基因与28月龄中国西门塔尔牛经济性状以及牛背最长肌脂肪酸组成之间的关系。结果表明，两个SNPs，即IVS3 + 478 C>T和IVS3 + 609 T>G，被确定与中国西门塔尔牛的胴体和肉质性状显著相关，如胴体脂肪覆盖率、眼肌面积和脂肪颜色评分。总之，我们的结果表明，该基因可影响牛的脂质代谢，并且这两个SNPs可能作为肉牛育种肉质性状的遗传标记。
