Department of Anatomy, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23 Rd, Bangkok, 10110, Thailand.
Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23 Rd, Bangkok, 10110, Thailand.
Probiotics Antimicrob Proteins. 2021 Feb;13(1):40-50. doi: 10.1007/s12602-020-09663-6.
Hepatic fibrosis is a reversible wound-healing response characterized by the accumulation of extracellular matrix. Probiotics have been used to prevent and treat various disorders. The aim of the present study was to investigate the hepatoprotective effects of probiotic lactic acid bacteria (mixture of Lactobacillus paracasei, Lactobacillus casei, and Weissella confusa) on thioacetamide (TAA)-induced liver fibrosis in rats. Thirty-five male Wistar rats were randomly divided into five groups: (1) control, (2) TAA, (3) TAA+probiotics, (4) TAA+silymarin, and (5) probiotics. Group 1 rats received a standard diet. In groups 2-4, fibrosis was induced by intraperitoneal injection of TAA (200 mg/kg BW) 3 times weekly for 8 consecutive weeks. Group 4 received TAA plus 100 mg/kg BW of silymarin 2 times weekly. Groups 3 and 5 were fed 10 CFU/mL viable microbial cells daily by gavage. The rats were sacrificed after 8 weeks of treatment. Liver tissues were collected immediately and processed for histopathological, lipid peroxidation, and Western blot analyses of TNF-α, TGF-β1, and α-SMA. Blood serum was collected to measure liver enzymes. Rats in the TAA groups suffered from hepatic injury (increased serum enzyme levels, liver inflammation, and increased concentration of TNF-α, TGF-β1, and α-SMA proteins) and extensive liver fibrosis. In contrast, TAA-treated rats receiving probiotics or silymarin had significantly lower serum enzyme levels, less inflammation, and less fibrosis. Liver damage was lower in the TAA+probiotics-treated group. Consumption of a mixture of probiotic lactic acid bacteria attenuates the development of liver fibrosis.
肝纤维化是一种可逆转的创伤愈合反应，其特征是细胞外基质的积累。益生菌已被用于预防和治疗各种疾病。本研究旨在探讨益生菌乳酸杆菌（副干酪乳杆菌、干酪乳杆菌和魏斯氏菌的混合物）对硫代乙酰胺（TAA）诱导的大鼠肝纤维化的保护作用。35 只雄性 Wistar 大鼠随机分为五组：（1）对照组，（2）TAA 组，（3）TAA+益生菌组，（4）TAA+水飞蓟素组，和（5）益生菌组。第 1 组大鼠给予标准饮食。在第 2-4 组中，通过每周 3 次腹腔注射 TAA（200mg/kg BW）连续 8 周诱导纤维化。第 4 组给予 TAA 加 100mg/kg BW 水飞蓟素，每周 2 次。第 3 和第 5 组通过灌胃给予 10 CFU/mL 活菌细胞。治疗 8 周后处死大鼠。立即采集肝组织，进行组织病理学、脂质过氧化和 TNF-α、TGF-β1 和 α-SMA 的 Western blot 分析。采集血清以测量肝酶。TAA 组大鼠发生肝损伤（血清酶水平升高、肝脏炎症和 TNF-α、TGF-β1 和 α-SMA 蛋白浓度增加）和广泛的肝纤维化。相比之下，TAA 处理的大鼠接受益生菌或水飞蓟素治疗后，血清酶水平、炎症和纤维化程度显著降低。TAA+益生菌治疗组的肝损伤程度较低。益生菌乳酸杆菌混合物的消耗可减轻肝纤维化的发展。
