Zhejiang Chinese Medical University, Hangzhou, 310057, PR China.
Food Funct. 2020 Jun 24;11(6):5389-5395. doi: 10.1039/d0fo00775g.
Hydroxycinnamic acids (HAs) are widely spread in food and herbal medicines. The bioavailability of HAs largely depends on the absorption and metabolism in enterohepatic circulation, in which gut microbiota plays a vital role. The present research aims to investigate the metabolism of HAs by gut microbiota, together with the community changes of gut microbiota after cerebral ischemia-reperfusion (I/R) injury. The results showed that non-substituted cinnamic acid (NCA) and sinapic acid (SA) were stable to gut microbiota, while 4-hydrocinnamic acid (4-HA), caffeic acid (CA), and ferulic acid (FA) underwent decarboxylation and hydrogenation after anaerobic incubation. Time-course studies indicated that the gut microbiota from I/R injured rats can catalyze the same reaction, but with a decreased reaction rate. The 16S rRNA sequencing technique was applied to uncover the community changes of gut microbiota. In addition, the metabolites of the three HAs exhibited greater activity in scavenging the DPPH radical and protected PC12 cells against oxidative damage.
羟基肉桂酸（HAs）广泛存在于食品和草药中。HAs 的生物利用度在很大程度上取决于肠肝循环中的吸收和代谢，而肠道微生物群在其中起着至关重要的作用。本研究旨在探讨肠道微生物群对 HAs 的代谢作用，以及脑缺血再灌注（I/R）损伤后肠道微生物群的群落变化。结果表明，未取代的肉桂酸（NCA）和芥子酸（SA）对肠道微生物群稳定，而 4-羟基肉桂酸（4-HA）、咖啡酸（CA）和阿魏酸（FA）在厌氧孵育后发生脱羧和加氢反应。时程研究表明，I/R 损伤大鼠的肠道微生物群可以催化相同的反应，但反应速率降低。16S rRNA 测序技术用于揭示肠道微生物群的群落变化。此外，三种 HAs 的代谢产物在清除 DPPH 自由基方面表现出更大的活性，并能保护 PC12 细胞免受氧化损伤。
