Department of Otorhinolaryngology, and Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, China.
Department of Otorhinolaryngology, and Head and Neck Surgery, Heilongjiang Provincial Hospital Affiliated to Harbin Institute of Technology, China.
Adv Clin Exp Med. 2020 May;29(5):525-534. doi: 10.17219/acem/118848.
Laryngeal cancer (LC) is one of the common malignant tumors in the head and neck area, and the survival rate for patients is low.
To investigate miR-122a and miR-3195 expressions in LC tissue, their correlations with clinicopathological features, and their impacts on Hep-2 proliferation and apoptosis.
Thirty LC and 20 peritumoral tissue specimens were analyzed. miR-122a, miR-122a-negative control sequence, miR-3195, and miR-3195-NG sequence were transfected into Hep-2 in the miR-122a-mimics, miR-122a-NG, miR-3195-mimics, and miR-3195-NG groups, respectively. The miR-122a-mimics-non-transfected and miR-3195-mimics-non-transfected groups used non-transfected Hep-2.
There were lower miR-122a, miR-3195 and occludin protein, and higher TBX1 protein expressions in LC than in the peritumoral tissue; the miR-122a level was associated with clinical stage (all p < 0.001). Positive correlations between miR-122a and miR-3195, and miR-122a and occludin expressions, and a negative correlation between miR-3195 and TBX1 expressions were observed (r = 0.418, r = 0.541, r = -0.428, all p < 0.001). The miR-122a and miR-3195 levels in the 2 mimics groups increased respectively compared to their NG and the non-transfected groups. At different time points after 24 h of transfection, the optical density in the 2 mimics groups was lower than in their NG groups. The miR-122a-mimics group had an increased occludin level and the miR-3195-mimics group had a decreased TBX1 level, and both groups had greater apoptosis rates than their NG groups and in the non-transfected groups (all p < 0.001).
miR-122a is associated with clinical stage. miR-122a and miR-3195 may act as tumor suppressors and play a role in LC pathogenesis. They can suppress Hep-2 proliferation and promote its apoptosis, probably owing to the upregulation of occludin by miR-122a and suppression of TBX1 by miR-3195.
喉癌（LC）是头颈部常见的恶性肿瘤之一，患者的生存率较低。
探讨 LC 组织中 miR-122a 和 miR-3195 的表达及其与临床病理特征的关系，以及对 Hep-2 增殖和凋亡的影响。
分析 30 例 LC 组织和 20 例癌旁组织标本。miR-122a、miR-122a 阴性对照序列、miR-3195 和 miR-3195-NG 序列分别转染至 Hep-2 中的 miR-122a-模拟物、miR-122a-NG、miR-3195-模拟物和 miR-3195-NG 组，miR-122a-模拟物-非转染组和 miR-3195-模拟物-非转染组使用非转染 Hep-2。
LC 组织中 miR-122a、miR-3195 和 occludin 蛋白表达降低，TBX1 蛋白表达升高；miR-122a 水平与临床分期有关（均 p < 0.001）。miR-122a 与 miR-3195、miR-122a 与 occludin 表达呈正相关，miR-3195 与 TBX1 表达呈负相关（r = 0.418、r = 0.541、r = -0.428，均 p < 0.001）。与 NG 组和非转染组相比，miR-122a 和 miR-3195 在 2 个模拟物组中的水平分别增加。转染 24 h 后不同时间点，2 个模拟物组的光密度值均低于其 NG 组。miR-122a 模拟物组 occludin 水平升高，miR-3195 模拟物组 TBX1 水平降低，与 NG 组和非转染组相比，这两组的凋亡率均升高（均 p < 0.001）。
miR-122a 与临床分期有关。miR-122a 和 miR-3195 可能作为肿瘤抑制因子发挥作用，参与 LC 的发病机制。它们可抑制 Hep-2 增殖并促进其凋亡，可能是通过 miR-122a 上调 occludin 和 miR-3195 下调 TBX1 实现的。
