Department of Marine Biotechnology Research Center, Korea Institute of Ocean Science and Technology, Busan 49111, South Korea; Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
Mar Pollut Bull. 2020 Jun;155:111062. doi: 10.1016/j.marpolbul.2020.111062. Epub 2020 May 11.
Global warming is a big concern for all organisms and many efforts have been made to reveal the potential effects of temperature elevation on aquatic organisms. However, limited studies on molecular mechanistic approaches on physiological effects due to temperature changes are available. Here, we investigated the effects of temperature changes on life parameters (e.g., population growth [total number of rotifers], and lifespan), oxidative stress levels and antioxidant activities (e.g., glutathione S-transferase [GST], catalase [CAT], superoxide dismutase [SOD]) with expression levels in the monogonont marine rotifer Brachionus plicatilis. The changes in temperatures led to significant reduction (P < 0.05) in lifespan, possibly due to significant decrease (P < 0.05) in antioxidant activities, reducing the potential to cope with significant elevation in the temperature-induced oxidative stress in B. plicatilis. To further assess the actual induction and clearance of reactive oxygen species (ROS), N-acetyl-L-cysteine was used to examine whether the temperature-induced oxidative stress could be successfully scavenged. Furthermore, expression patterns of the antioxidant-related genes (GSTs, SODs, and CATs) were down- or upregulated (P < 0.05) in response to different temperatures in B. plicatilis. Overall, these findings indicate that ROS-mediated oxidative stress led to cellular damage and antioxidant defense system, resulting in deleterious effects on life parameters in rotifer.
全球变暖是所有生物面临的一个重大问题，人们已经做出了许多努力来揭示温度升高对水生生物的潜在影响。然而，由于温度变化对生理效应的分子机制方法的研究有限。在这里，我们研究了温度变化对生命参数（如种群增长率[轮虫总数]和寿命）、氧化应激水平和抗氧化活性（如谷胱甘肽 S-转移酶[GST]、过氧化氢酶[CAT]、超氧化物歧化酶[SOD]）以及海洋单巢轮虫褶皱臂尾轮虫表达水平的影响。温度的变化导致寿命显著减少（P<0.05），这可能是由于抗氧化活性显著下降（P<0.05），降低了轮虫应对温度升高引起的氧化应激的潜力。为了进一步评估活性氧（ROS）的实际诱导和清除，使用 N-乙酰-L-半胱氨酸来检查温度诱导的氧化应激是否可以被成功清除。此外，抗氧化相关基因（GSTs、SODs 和 CATs）的表达模式在褶皱臂尾轮虫中响应不同的温度而被下调或上调（P<0.05）。总的来说，这些发现表明 ROS 介导的氧化应激导致细胞损伤和抗氧化防御系统，从而对轮虫的生命参数产生有害影响。
