通过芯片分析鉴定和分析煤焦油沥青诱导恶性转化的 BEAS-2B 细胞中的关键 lncRNAs。
Identification and analysis of key lncRNAs in malignant-transformed BEAS-2B cells induced with coal tar pitch by microarray analysis.
机构信息
Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou, Henan province, 450001, China.
Rizhao Center for Disease Control and Prevention, Rizhao, Shandong province, 276800, China.
出版信息
Environ Toxicol Pharmacol. 2020 Oct;79:103376. doi: 10.1016/j.etap.2020.103376. Epub 2020 May 12.
This study aims to explore the key and differentially expressed long non-coding RNAs (lncRNAs) and elucidates their possible mechanisms in malignant-transformed Human bronchial epithelial (BEAS-2B) cells induced by coal tar pitch extracts (CTPE). BEAS-2B cells were stimulated with 2.4 μg/ml CTPE, then passaged for three times which were named CTPE1 and then passaged until passage 30 (CTPE30). The results showed that cells of CTPE30 appeared abnormal morphology. Furthermore, migration, clonality and proliferation of cells in CTPE group were significantly increased compared with those in control groups. However, the apoptosis of cells in CTPE group was inhibited. A total of 569 differentially expressed mRNAs and 707 differentially expressed lncRNAs were screened out, among which four lncRNAs were validated and were consistent with the microarray results. 32 target genes were screened out by Co-expression network. The study suggests that differentially expressed lncRNAs may play a potential role in lung carcinogenesis.
本研究旨在探索煤焦油沥青提取物（CTPE）诱导恶性转化的人支气管上皮（BEAS-2B）细胞中的关键差异表达长非编码 RNA（lncRNA），并阐明其可能的机制。用 2.4μg/ml CTPE 刺激 BEAS-2B 细胞，然后传代 3 次，分别命名为 CTPE1，然后传代至 30 代（CTPE30）。结果表明，CTPE30 组细胞出现异常形态。此外，与对照组相比，CTPE 组细胞的迁移、克隆形成和增殖能力显著增强，而细胞凋亡受到抑制。筛选出 569 个差异表达的 mRNAs 和 707 个差异表达的 lncRNAs，其中 4 个 lncRNAs经验证与微阵列结果一致。通过共表达网络筛选出 32 个靶基因。研究表明，差异表达的 lncRNAs 可能在肺癌发生中发挥潜在作用。
Zotero 插件