一种基于双（萨拉莫）的高选择性化学传感器的合成、表征及活细胞成像
Synthesis and characterization for a highly selective bis(salamo)-based chemical sensor and imaging in living cell.
作者信息
Wang Lan, Wei Zhi-Li, Liu Chang, Dong Wen-Kui, Ru Jia-Xi
机构信息
School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, China.
School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 5;239:118496. doi: 10.1016/j.saa.2020.118496. Epub 2020 May 16.
A new sensor HL for continuous identification of Cu, Al and lysine was synthesized by Schiff base reactions. The sensor could specifically recognized Cu in the EtOH/HO (1:1 v/v) solution by UV-vis spectra, and the binding constant with Cu can reach 10 M, meanwhile, it was found by the naked-eye that the color of the solution was changed from colorless to yellow. The copper complex L-Cu formed by the sensor HL and Cu could further recognize Al and lysine in the fluorescence spectra. The LOD values of the three objects were 2.67 × 10, 1.96 × 10 and 5.59 × 10 M, respectively. In addition, fluorescence intracellular images of Al and lysine were performed and obtained satisfactory results.
通过席夫碱反应合成了一种用于连续识别铜、铝和赖氨酸的新型传感器HL。该传感器可通过紫外可见光谱在乙醇/水（1:1 v/v）溶液中特异性识别铜，与铜的结合常数可达10⁶ M，同时肉眼可观察到溶液颜色由无色变为黄色。传感器HL与铜形成的铜配合物L-Cu可在荧光光谱中进一步识别铝和赖氨酸。这三种物质的检测限分别为2.67×10⁻⁷、1.96×10⁻⁷和5.59×10⁻⁶ M。此外，还进行了铝和赖氨酸的细胞内荧光成像并获得了满意的结果。
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