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一种用于选择性检测食品样品和细胞中L-赖氨酸的新型硼酸基荧光传感器。

A novel boronic acid-based fluorescent sensor for the selective detection of l-lysine in food samples and cells.

作者信息

Gao Wei, Su Jing, Yang Huarui, Zhao Xuefeng, Liu Jingjing, Wang Zhijun, Wang Qingming

机构信息

Department of Chemistry, Changzhi University Changzhi 046011 People's Republic of China.

School of Pharmacy, Yancheng Teachers' University Yancheng 224051 People's Republic of China.

出版信息

RSC Adv. 2025 Apr 4;15(13):10453-10459. doi: 10.1039/d5ra00621j. eCollection 2025 Mar 28.

DOI:10.1039/d5ra00621j
PMID:40191141
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11969143/
Abstract

A novel probe DFC (2-(dicyanomethylene)-2,5-dihydro-5,5-dimethyl-4-(()-2-(5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)furan-2-yl)vinyl)furan-3-carbonitrile) was successfully designed and synthesized for the detection of l-lysine (l-Lys). The sensing behavior was characterized using absorption and fluorescence emission spectra. Upon addition of l-Lys to DFC, a rapid response time of 5 seconds was observed, accompanied by a significant 4-fold enhancement in fluorescence intensity. Additionally, DFC exhibits an impressively low detection limit of 0.14 μMol L. Furthermore, the applicability of DFC was demonstrated through successful detection of l-Lys in water samples, food samples, and imaging of l-lys in live HeLa cells.

摘要

成功设计并合成了一种新型探针DFC（2-（二氰基亚甲基）-2,5-二氢-5,5-二甲基-4-（（）-2-（5-（4,4,5,5-四甲基-1,3,2-二氧杂硼烷-2-基）呋喃-2-基）乙烯基）呋喃-3-甲腈）用于检测L-赖氨酸（L-Lys）。利用吸收光谱和荧光发射光谱对传感行为进行了表征。向DFC中加入L-Lys后，观察到快速响应时间为5秒，同时荧光强度显著增强了4倍。此外，DFC表现出令人印象深刻的低检测限，为0.14μMol/L。此外，通过成功检测水样、食品样品中的L-Lys以及对活HeLa细胞中的L-赖氨酸进行成像，证明了DFC的适用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/9606d29114c0/d5ra00621j-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/43f6632ed310/d5ra00621j-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/3038d863bf02/d5ra00621j-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/5ec97423fb62/d5ra00621j-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/d0965b47e682/d5ra00621j-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/ce6e00f733ea/d5ra00621j-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/0c516c282b2a/d5ra00621j-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/bd251c14b3d2/d5ra00621j-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/2e7ec7ec87e0/d5ra00621j-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/9606d29114c0/d5ra00621j-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/43f6632ed310/d5ra00621j-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/3038d863bf02/d5ra00621j-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/5ec97423fb62/d5ra00621j-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/d0965b47e682/d5ra00621j-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/ce6e00f733ea/d5ra00621j-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/0c516c282b2a/d5ra00621j-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/bd251c14b3d2/d5ra00621j-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/2e7ec7ec87e0/d5ra00621j-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c32/11969143/9606d29114c0/d5ra00621j-f7.jpg

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