College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China; Institute of Biology, Freie Universität Berlin Altensteinstr. 6, 14195 Berlin, Germany.
Department of Agricultural and Biological Engineering, University of Florida, Gainesville, FL 32611, United States.
Bioresour Technol. 2020 Sep;311:123584. doi: 10.1016/j.biortech.2020.123584. Epub 2020 May 26.
The transcription and expression of functional genes governed the biotransformation of nutrients during composting. In this study, the diversities of functional genes were investigated in 50 days chicken manure composting through six treatments amended with different dosage of red kaolin. Results showed that based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation, Metabolism possessed largest proportion and richest genes number among six pathways, of which carbohydrate metabolism (81007) were significantly greater than others; while Human Disease and Organismal Systems were relatively lower. Meanwhile, the addition of red kaolin has an important impact on dynamics of KEGG genes, the relative abundance of detected genes were distinctively different in all treatments. Similarity analysis also showed the varying influence of different proportion of red kaolin on functional genes during chicken manure composting. In conclusion, application of red kaolin in chicken manure composting effectively improved the relative abundance of functional genes.
功能基因的转录和表达控制着堆肥过程中营养物质的生物转化。本研究通过向鸡粪堆肥中添加不同剂量的红黏土，对 50 天鸡粪堆肥过程中的功能基因多样性进行了研究。结果表明，基于京都基因与基因组百科全书（KEGG）通路注释，在六个通路中，代谢途径具有最大的比例和最丰富的基因数量，其中碳水化合物代谢（81007）显著大于其他途径；而人类疾病和机体系统相对较低。同时，红黏土的添加对 KEGG 基因的动态有重要影响，所有处理中检测到的基因的相对丰度明显不同。相似性分析还表明，不同比例的红黏土对鸡粪堆肥过程中功能基因的影响不同。总之，在鸡粪堆肥中应用红黏土可以有效提高功能基因的相对丰度。
