使用一种拟益生菌和后生元饲料补充剂（HWF™）可改善杂交鲟（施氏鲟×西伯利亚鲟）的生长性能、肠道微生物组成和功能。
Use of a paraprobiotic and postbiotic feed supplement (HWF™) improves the growth performance, composition and function of gut microbiota in hybrid sturgeon (Acipenser baerii x Acipenser schrenckii).
机构信息
Guizhou University, Guiyang, 550025, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha, 410081, China.
China-Norway Joint Lab on Fish Gastrointestinal Microbiota, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Tigray Agricultural Research Institute, Mekelle, Tigray, Ethiopia.
出版信息
Fish Shellfish Immunol. 2020 Sep;104:36-45. doi: 10.1016/j.fsi.2020.05.054. Epub 2020 May 27.
The contribution of cold water aquaculture for the world fish production is significant. Although sturgeon farming is an important part of China's cold water aquaculture industry, its production is less compared with the current potentiality of the country. There are many reasons for the lower production of cold water fish farming including feed and disease. The aim of the present study was to investigate the effect of a paraprobiotic and postbiotic feed supplement (Herpes Worry Free or HWF™) on the growth, feeding efficiency and gut microbiota balance of hybrid sturgeon. Besides, the effect of sturgeon gut microbiota fed on the diet supplemented with HWF™ on the expression of growth promoter, and immune regulatory genes of germ free (GF) zebrafish was evaluated. Sturgeon were fed for three weeks with HWF™ supplemented or basal diet. At the end of the experiment gut content of sturgeon, fed on either experimental diet was transferred and colonized to GF zebrafish. Sturgeon fed with HWF™ supplemented diet showed significantly higher weight gain rate and lower feed conversion ratio (FCR) as compared with the control (P < 0.05). Compared with the control group, the relative abundance of Firmicutes, were significantly higher in the HWF™ group (P < 0.05), whereas Proteobacteria, Actinobacteria and Chlamydiae were significantly higher in the control group (P < 0.05). Furthermore, at the genus level Clostridium (64.50 ± 5.99%) and Lactococcus (29.5 ± 3.05%) were the most dominant gut bacteria in the HWF™ group and the control group of sturgeon, respectively. The expression of genes related to growth, inflammation and non-specific immunity was significantly upregulated in GF zebrafish colonized with gut microbiota of HWF™ sturgeon group. In conclusion, HWF™ played significant role in growth, feed efficiency and modulation of gut microbiota of sturgeon. The gut microbiota of sturgeon fed on the diet supplemented with HWF™ upregulated the expression of genes related to growth, inflammation and non-specific immunity in GF zebrafish model.
冷水水产养殖对世界鱼类产量的贡献意义重大。尽管鲟鱼养殖是中国冷水水产养殖业的重要组成部分，但与国家目前的潜力相比，其产量较低。冷水鱼类养殖产量较低的原因有很多，包括饲料和疾病。本研究旨在探讨一种益生菌和后生元饲料补充剂（疱疹无忧或 HWF™）对杂交鲟鱼生长、摄食效率和肠道微生物平衡的影响。此外，还评估了鲟鱼肠道微生物群在补充 HWF™的饮食上对无特定病原体（GF）斑马鱼生长促进和免疫调节基因表达的影响。鲟鱼用 HWF™补充或基础饲料喂养三周。实验结束时，将两种实验饲料喂养的鲟鱼的肠道内容物转移并定植到 GF 斑马鱼体内。与对照组相比，用 HWF™补充饲料喂养的鲟鱼的体重增长率显著提高，饲料转化率（FCR）显著降低（P<0.05）。与对照组相比，HWF™组的厚壁菌门的相对丰度显著更高（P<0.05），而对照组的变形菌门、放线菌门和衣原体门的相对丰度显著更高（P<0.05）。此外，在属水平上，HWF™组的梭菌（64.50±5.99%）和乳球菌（29.5±3.05%）是鲟鱼肠道中最主要的细菌，而对照组的鲟鱼肠道中最主要的细菌是梭菌（64.50±5.99%）和乳球菌（29.5±3.05%）。HWF™组鲟鱼的肠道微生物群定植在 GF 斑马鱼体内后，与生长、炎症和非特异性免疫相关的基因表达显著上调。综上所述，HWF™在鲟鱼的生长、摄食效率和肠道微生物群调节方面发挥了重要作用。补充 HWF™的饲料喂养的鲟鱼的肠道微生物群上调了 GF 斑马鱼模型中与生长、炎症和非特异性免疫相关的基因表达。
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