Ekasari Julie, Mirza Vini Nur, Fauzi Ichsan Achmad, Hashimoto Kyohei, Suprayudi Muhammad Agus
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, Indonesia.
House Wellness Foods Corporation, Hyogo, Japan.
Aquac Nutr. 2023 Aug 22;2023:8860364. doi: 10.1155/2023/8860364. eCollection 2023.
Thus, this research was conducted to evaluate the supplementation of heat-killed at higher dosages and investigate the effect of heat-killed supplementation on the challenges of . A feeding trial was conducted for 90 (initial average body weight of 12.52-12.69 g) days, while a disease challenge was conducted for 17 days. Dietary treatments were formulated to have a ranging level of heat-killed L-137: (1) control treatment without heat-killed , (2) diet containing 10 mg/kg heat-killed L-137 preparation (LP20, which contains 20% heat-killed L-137), (3) diet containing 20 mg/kg LP20, (4) diet containing 100 mg/kg LP20, and (5) diet containing 250 mg/kg LP20. All the diets were formulated to have equal values of protein and energy. Dietary supplementation of heat-killed L-137 improved tilapia growth performance and higher robustness against . infection. Therefore, a 10-20 mg/kg LP20 feed supplementation level is recommended to support the tilapia growth. In addition, an LP20 dietary supplementation level of 250 mg/kg feed is recommended for higher protection against .
因此，本研究旨在评估高剂量热灭活[具体物质未明确，推测是某种益生菌之类，用“[具体物质]”代替]的添加情况，并研究热灭活[具体物质]添加对[具体挑战未明确，推测是某种疾病之类，用“[具体挑战]”代替]挑战的影响。进行了为期90天（初始平均体重12.52 - 12.69克）的饲养试验，同时进行了为期17天的疾病挑战。日粮处理配方设计为含有不同水平的热灭活[具体物质]L - 137：（1）不添加热灭活[具体物质]的对照处理；（2）含有10毫克/千克热灭活[具体物质]L - 137制剂（LP20，其中含有20%热灭活[具体物质]L - 137）的日粮；（3）含有20毫克/千克LP20的日粮；（4）含有100毫克/千克LP20的日粮；（5）含有250毫克/千克LP20的日粮。所有日粮的蛋白质和能量值均相等。日粮中添加热灭活[具体物质]L - 137可提高罗非鱼的生长性能，并增强其对[具体挑战]感染的抵抗力。因此，建议添加10 - 20毫克/千克LP20的饲料水平以支持罗非鱼生长。此外，建议日粮中添加250毫克/千克饲料的LP20水平以获得更高的对[具体挑战]的保护。
