Microbial sulfate reduction facilitates seasonal variation of arsenic concentration in groundwater of Jianghan Plain, Central China.

Bacterial sulfate reduction (BSR) plays a vital but complex role in regulating groundwater arsenic concentration. A quarterly hydro-biogeochemical investigation was conducted to clarify how BSR participated in arsenic dynamics in the geogenic As-contaminated alluvial aquifers of the Jianghan Plain, central Yangtze River Basin. Anthropogenic input of sulfate was identified in the transitional season with higher Cl concentrations and Cl/Br molar ratios compared to the monsoon season. Seasonal increase of S(-II) and Fe(II) concentrations in monsoon season suggests the co-occurrence of iron and sulfate reduction. Quantitative analysis of dsrB gene abundance revealed the corresponding variations between dsrB gene abundance (up to 1.2 × 10 copies L) and Fe(II) in groundwater. High-throughput sequencing of the dsrB gene identified a considerable proportion of sequences in the sulfate-reducing bacterial community was affiliated with Desulfobulbus (22.7 ± 20.8%) and Desulfocapsa (11.5 ± 11.9%). Moreover, the relative abundance of Desulfocapsa increased with the Fe(II) in the groundwater (R = 0.78, P < 0.01). These results suggest that microbially-mediated sulfate reduction facilitated the abiotic reduction of As-bearing Fe-oxides in the monsoon season after anthropogenic input of sulfate in the transitional season under oscillating redox conditions in the groundwater systems. The present research provides new insights into the critical role of BSR in the seasonal redox cycling of iron and variation of As in the aquifer systems, which are not only applicable in the central Yangtze River basin, but also to other similar As-rich alluvial aquifers worldwide.