miR-125 通过调控 VEGF 和 PI3K/AKT 信号通路抑制宫颈癌进展。
MiR-125 inhibited cervical cancer progression by regulating VEGF and PI3K/AKT signaling pathway.
机构信息
Department of Gynecology and Obstetrics, The Fourth People's Hospital of Liaocheng, City, Shandong Province, Liaocheng, China.
Department of Gynecology and Obstetrics, Liaocheng People's Hospital, Liaocheng City, Shandong Province, China.
出版信息
World J Surg Oncol. 2020 May 30;18(1):115. doi: 10.1186/s12957-020-01881-0.
BACKGROUND
MiR-125 has been shown to be involved in a variety of cancers, including cervical cancer (CC). Here, our goal was to explore miR-125 functional role and molecular mechanism in cervical cancer development and progression.
METHODS
qRT-PCR was employ to detect miR-125 and VEGF mRNA expression. Western blot was applied for testing protein levels (VEGF, E-cadherin, N-cadherin, vimentin, AKT, p-AKT, PI3K, and p-PI3K). MTT and transwell assays were used for detecting cervical cancer cell progression, including cell viability, migration, and invasion.
RESULTS
We observed that miR-125 was downregulated, whereas VEGF was upregulated in cervical cancer tissues and cell lines (CaSki and SiHa). MiR-125 inhibited the proliferation, invasion, and migration by targeting VEGF in cervical cancer. Moreover, miR-125 negatively regulated VEGF expression in cervical cancer tissues. Finally, we demonstrated that miR-520d-5p inhibited the activation of PI3K/AKT signaling pathway.
CONCLUSION
In conclusion, the findings demonstrated that miR-125 inhibited cervical cancer progression and development by suppression VEGF and PI3K/AKT signaling pathway.
背景
miR-125 已被证明参与多种癌症，包括宫颈癌（CC）。在这里，我们的目标是探讨 miR-125 在宫颈癌发生和发展中的功能作用和分子机制。
方法
采用 qRT-PCR 检测 miR-125 和 VEGF mRNA 表达。Western blot 用于检测蛋白水平（VEGF、E-cadherin、N-cadherin、vimentin、AKT、p-AKT、PI3K 和 p-PI3K）。MTT 和 Transwell 检测用于检测宫颈癌细胞的进展，包括细胞活力、迁移和侵袭。
结果
我们观察到 miR-125 在宫颈癌组织和细胞系（CaSki 和 SiHa）中下调，而 VEGF 上调。miR-125 通过靶向 VEGF 抑制宫颈癌的增殖、侵袭和迁移。此外，miR-125 负调控宫颈癌组织中 VEGF 的表达。最后，我们证明 miR-520d-5p 抑制了 PI3K/AKT 信号通路的激活。
结论
综上所述，研究结果表明 miR-125 通过抑制 VEGF 和 PI3K/AKT 信号通路抑制宫颈癌的进展和发展。
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