Department of Gynaecology, Maternal and Child Health Hospital of HuBei Province, Wuhan, China.
Eur Rev Med Pharmacol Sci. 2019 Jan;23(2):513-521. doi: 10.26355/eurrev_201901_16863.
Deregulation of microRNAs (miRNAs) has been identified as critical event in tumor initiation and progression. We aimed to explore the role of miR-374b in cervical cancer progression.
MiR-374b expression was detected using qRT-PCR in cervical cancer tissues compared with normal counterparts. Cell proliferation and invasion ability were detected using Cell Counting Kit-8 (CCK8) cell proliferation and transwell invasion assay. Dual luciferase reporter assay, qRT-PCR, and Western blot analysis were used to demonstrate that FOXM1 was a target of miR-374b.
We demonstrated that downregulation of miR-374b was frequently examined in cervical cancer tissues compared with normal counterparts. Furthermore, we showed the lower miR-374b expression associated with lymph node metastasis and advanced FIGO stage in patients with cervical cancer. Furthermore, ectopic expression of miR-374b could significantly decrease cell proliferation and invasion ability. However, inhibition of miR-374b had opposite effects. Dual luciferase reporter assay, qRT-PCR, and Western blot analysis revealed that miR-374b overexpression suppressed cell proliferation and invasion ability via affecting FOXM1 expression.
These results indicated that miR-374b acted as tumor suppressor and may serve as a potential target for cervical cancer treatment.
microRNAs（miRNAs）的失调已被确定为肿瘤发生和进展的关键事件。我们旨在探讨 miR-374b 在宫颈癌进展中的作用。
采用 qRT-PCR 检测宫颈癌组织与正常对照中 miR-374b 的表达。采用细胞计数试剂盒-8（CCK8）细胞增殖和 Transwell 侵袭实验检测细胞增殖和侵袭能力。双荧光素酶报告基因实验、qRT-PCR 和 Western blot 分析用于证明 FOXM1 是 miR-374b 的靶基因。
我们证明与正常对照相比，miR-374b 在宫颈癌组织中经常下调。此外，我们发现 miR-374b 表达水平较低与宫颈癌患者的淋巴结转移和 FIGO 晚期分期相关。此外，外源性表达 miR-374b 可显著降低细胞增殖和侵袭能力。然而，抑制 miR-374b 则有相反的效果。双荧光素酶报告基因实验、qRT-PCR 和 Western blot 分析表明，miR-374b 通过影响 FOXM1 表达来抑制细胞增殖和侵袭能力。
这些结果表明，miR-374b 作为肿瘤抑制因子发挥作用，可能成为宫颈癌治疗的潜在靶点。
