苦参碱通过下调ERK1/2信号通路抑制HepG2细胞的增殖和迁移。
Matrine inhibits proliferation and migration of HepG2 cells by downregulating ERK1/2 signaling pathways.
作者信息
Yu Haipeng, Zhu Bolan, Yang Wan, Zhang Qian, Wang Wei, Zhai Weichun, Lu Lixia, Zheng Ying, Dang Zheng, Li Bin, Li Chuyi, Liang Bin, Zhang Jiucong, Yu Xiaohui
机构信息
Department of Interventional Therapy, Tianjin Medical University Cancer Institute and Hospital; National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer; Key Laboratory of Cancer Prevention and Therapy, Tianjin, China.
Department of Gastroenterology, The 940 Hospital of Joint Logistic Support Force of People's Liberation Army, Lanzhou, China.
出版信息
J Cancer Res Ther. 2020;16(2):209-214. doi: 10.4103/jcrt.JCRT_331_19.
OBJECTIVE
To research the effect of matrine on the proliferation and migration of HepG2 cells through extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway.
METHODS
HepG2 cell was selected and divided into blank control group, experimental group (matrine 1, 2, and 4 mg/mL), and positive control group (PD98059, ERK1/2 inhibitor). MTT measure was used to detect the effective time and concentration which matrine inhibits HepG2 cells. After 24 h, the effect of effective concentration of matrine on the of morphological changing HepG2 cells was observed. The invasion ability was assayed by transwell method, the expression of ERK1/2 and pERK1/2 were detected through Western blot, and reverse transcription polymerase chain reaction was used to test the expression level of ERK1/2 mRNA.
RESULTS
With the increase of matrine concentration, the number of adherent HepG2 cells gradually decreased, the morphologic changes gradually became spherical, some cell morphology was incomplete, and even cell fragments appeared. The proliferation and invasion ability of HepG2 cells decreased. The expression of ERK1/2, pERK1/2, and ERK1/2 mRNA downregulated with the increase of matrine concentration (P < 0.05).
CONCLUSION
Matrine inhibits the proliferation and migration of HepG2 cells by downregulating the ERK1/2 signaling pathway.
目的
研究苦参碱通过细胞外信号调节激酶1/2（ERK1/2）信号通路对HepG2细胞增殖和迁移的影响。
方法
选取HepG2细胞，分为空白对照组、实验组（苦参碱1、2和4 mg/mL）和阳性对照组（PD98059，ERK1/2抑制剂）。采用MTT法检测苦参碱抑制HepG2细胞的有效时间和浓度。24 h后，观察有效浓度苦参碱对HepG2细胞形态变化的影响。采用transwell法检测侵袭能力，通过蛋白质免疫印迹法检测ERK1/2和pERK1/2的表达，并采用逆转录聚合酶链反应检测ERK1/2 mRNA的表达水平。
结果
随着苦参碱浓度的增加，贴壁的HepG2细胞数量逐渐减少，形态变化逐渐呈球形，部分细胞形态不完整，甚至出现细胞碎片。HepG2细胞的增殖和侵袭能力下降。随着苦参碱浓度的增加，ERK1/2、pERK1/2和ERK1/2 mRNA的表达下调（P < 0.05）。
结论
苦参碱通过下调ERK1/2信号通路抑制HepG2细胞的增殖和迁移。
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