人精液中18-羟基前列腺素E1和E2的分离与生物合成

Isolation and biosynthesis of 18-hydroxyprostaglandins E1 and E2 in human seminal fluid.

作者信息

Oliw E H

机构信息

Department of Pharmacology, Karolinska institutet, Stockholm, Sweden.

出版信息

Prostaglandins. 1988 Apr;35(4):523-33. doi: 10.1016/0090-6980(88)90028-7.

DOI:10.1016/0090-6980(88)90028-7

PMID:3247470

Abstract

18-Hydroxy-PGE1 and 18-hydroxy-PGE2 were identified in human seminal fluid by capillary gas-liquid chromatography-mass spectrometry. The levels of these prostaglandins was 1-2% of the corresponding 19-hydroxy-PGE compounds in human semen. 18-Hydroxy-PGE1 and 18-hydroxy-PGE2 are likely formed by cytochrome P-450 in seminal vesicles in analogy with the 19-hydroxy-PGE compounds. This was supported by the finding that microsomes of seminal vesicles of the cynomolgus monkey, Macaca fascicularis, supplemented with 1 mM NADPH, metabolized PGE1 to both 19-hydroxy-PGE1 (92%) and 18-hydroxy-PGE1 (8%). The hydroxylation of prostaglandins in seminal vesicles of primates may thus show a high but not absolute specificity for the penultimate carbon of prostaglandins.

摘要

通过毛细管气-液色谱-质谱联用技术在人精液中鉴定出了18-羟基前列腺素E1和18-羟基前列腺素E2。这些前列腺素的水平是人精液中相应的19-羟基前列腺素化合物的1%-2%。18-羟基前列腺素E1和18-羟基前列腺素E2可能是由精囊中的细胞色素P-450形成的，类似于19-羟基前列腺素化合物。食蟹猴（猕猴）精囊微粒体在添加1 mM烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的情况下将前列腺素E1代谢为19-羟基前列腺素E1（92%）和18-羟基前列腺素E1（8%），这一发现支持了上述观点。因此，灵长类动物精囊中前列腺素的羟基化可能对前列腺素的倒数第二个碳原子表现出高但非绝对的特异性。