在公羊体内一种新型前列腺素δ17 - PGE1的生物合成。

Biosynthesis of a novel prostaglandin, delta 17-PGE1, in the ram.

作者信息

Oliw E H, Sprecher H, Hamberg M

出版信息

Acta Physiol Scand. 1986 May;127(1):45-9. doi: 10.1111/j.1748-1716.1986.tb07874.x.

DOI:10.1111/j.1748-1716.1986.tb07874.x

Abstract

The prostaglandin, delta 17-PGE1, was purified from extracts of ram seminal fluid by reversed phase high performance liquid chromatography (HPLC) and identified after conversion to delta 17-PGB1 by UV-analysis and by capillary column gas chromatography-mass spectrometry (GC-MS). It was also formed by incubation of a homogenate of ram vesicular glands. The amount of delta 17-PGE1 in the seminal fluid and in the homogenate averaged 12% and 25% of the amount of PGE2, respectively. The results show that cis-8,11,14,17-eicosatetraenoic acid can be metabolized to prostaglandins in vivo in the ram.

摘要

前列腺素δ17-PGE1通过反相高效液相色谱法（HPLC）从公羊精液提取物中纯化出来，并在通过紫外分析和毛细管柱气相色谱-质谱联用仪（GC-MS）转化为δ17-PGB1后进行鉴定。它也通过公羊精囊匀浆孵育形成。精液和匀浆中δ17-PGE1的量分别平均为PGE2量的12%和25%。结果表明，顺式-8,11,14,17-二十碳四烯酸在公羊体内可在体内代谢为前列腺素。