培养和操作小鼠胚胎干细胞。

Culturing and Manipulating Mouse Embryonic Stem Cells.

机构信息

Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA.

出版信息

Methods Mol Biol. 2020;2155:1-9. doi: 10.1007/978-1-0716-0655-1_1.

Abstract

Mouse embryonic stem cells (mESC) have the ability to self-renew due to their rapid proliferation and high telomerase activity while maintaining their pluripotency. Depending on the environment, mESC can differentiate into a broad range of cell types. These characteristics have established mESC as a tool for modeling human disease, genetic engineering, lineage specificity, stem cell-based therapies, and tissue regeneration. Here we describe a protocol for mESC expansion and differentiation.

摘要

小鼠胚胎干细胞（mESC）由于其快速增殖和高端粒酶活性，具有自我更新的能力，同时保持其多能性。根据环境的不同，mESC 可以分化为广泛的细胞类型。这些特性使 mESC 成为建模人类疾病、基因工程、谱系特异性、基于干细胞的治疗和组织再生的工具。在这里，我们描述了一个 mESC 扩增和分化的方案。