牛、鼠和人精原干细胞的分离、培养、冷冻保存和鉴定。

Isolation, Culture, Cryopreservation, and Identification of Bovine, Murine, and Human Spermatogonial Stem Cells.

机构信息

Colegio de Ciencias Biológicas y Ambientales, Universidad San Francisco de Quito (USFQ), Quito, Ecuador.

Colegio de Ciencias de la Salud, Escuela de Medicina Veterinaria, Universidad San Francisco de Quito (USFQ), Quito, Ecuador.

出版信息

Methods Mol Biol. 2020;2155:151-164. doi: 10.1007/978-1-0716-0655-1_13.

DOI:10.1007/978-1-0716-0655-1_13

PMID:32474875

Abstract

Spermatogonial stem cells (SSCs) are the germ cells at the basis of spermatogenesis in adult mammals. SSCs offer many biotechnological possibilities and are fundamental cells in the study of spermatogenesis (Aponte, World J Stem Cells 7:669-680, 2015). This chapter describes detailed procedures for SSC isolation, culture, cryopreservation, and characterization in bovine, murine, and human models.

摘要

精原干细胞（SSCs）是成年哺乳动物精子发生的基础生殖细胞。SSCs 提供了许多生物技术的可能性，并且是精子发生研究的基础细胞（Aponte，《世界干细胞杂志》7:669-680, 2015）。本章描述了在牛、鼠和人模型中 SSC 分离、培养、冷冻保存和鉴定的详细程序。