College of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China.
Chongqing Academy of Chinese Materia Medica, Nanshan Road 34, Chongqing 400065, PR China.
Carbohydr Polym. 2020 Jul 15;240:116271. doi: 10.1016/j.carbpol.2020.116271. Epub 2020 Apr 25.
This study was designed to explore the effect of Chitosan oligosaccharides (COS) on mouse hindlimb ischemia by femoral artery ligation. Here, we demonstrated that COS treatment statistically promoted the blood perfusion and neovascularization in ischemic hindlimb of mice, accompanied by the suppression of inflammation and oxidative stress. By 16S rDNA gene sequencing, the disbalanced gut microbiota was observed in ischemic mice, while COS treatment, at least in part, restored the abundance changes of some intestinal bacteria at either phylum or genus levels. Based on metabolomics analysis on mouse plasma by UPLC-QTOF-MS, we screened 20 metabolites with the largest responses to ischemia, several of which were markedly reversed by COS. By Spearman's correlation analysis, the changed metabolites might act as a bridge between improved intestinal bacterial structure and alleviated hindlimb ischemia of mice treated by COS. Our studies point towards a potential role of COS in treatment of peripheral ischemia diseases.
本研究旨在通过股动脉结扎探讨壳寡糖（COS）对小鼠后肢缺血的影响。结果表明，COS 处理可显著促进小鼠缺血后肢的血液灌注和新生血管形成，同时抑制炎症和氧化应激。16S rDNA 基因测序显示，缺血小鼠的肠道微生物群失衡，而 COS 处理至少部分恢复了某些肠细菌在门或属水平的丰度变化。通过 UPLC-QTOF-MS 对小鼠血浆进行代谢组学分析，筛选出对缺血反应最大的 20 种代谢物，其中几种代谢物经 COS 处理后明显逆转。通过 Spearman 相关分析，这些变化的代谢物可能在改善肠道细菌结构和缓解 COS 治疗后小鼠后肢缺血之间起到桥梁作用。我们的研究表明 COS 在治疗周围缺血性疾病方面具有潜在作用。
