Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan.
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan.
Biochem Biophys Res Commun. 2020 Jul 12;528(1):193-198. doi: 10.1016/j.bbrc.2020.04.128. Epub 2020 May 28.
Mangosteen, a fruit mainly produced in Southeast Asia, has been used as food and as an antipyretic and for treating skin diseases. The xanthones contained in mangosteen have many physiological activities including melanin suppression and anticancer activities, but little is known about the physiological effects of the most abundant xanthone, α-mangostin (α-MG) on myoblasts. In this study, we applied α-MG to C2C12 cells that had been induced to differentiate using 2% HS, and analyzed the physiological action of α-MS and the underlying mechanism in the context of myogenic differentiation. α-MG increased the survival rate of C2C12 cells in a concentration-dependent manner. Analysis of the morphological changes in the cells showed that α-MG significantly enhanced the myogenic differentiation of C2C12 myoblasts, whereas the mitochondrial number was only slightly affected. Expression analysis of differentiation-related proteins in C2C12 cells revealed that α-MG promoted the expression of MyoD and Myogenin. Thus, the present study revealed that α-MG improves the survival and myogenic differentiation of C2C12 myoblasts.
山竹果主要产于东南亚地区，既可食用，也可退热，还可治疗皮肤病。山竹果中的黄烷酮具有多种生理活性，包括抑制黑色素生成和抗癌活性，但对含量最丰富的黄烷酮 α-倒捻子素（α-MG）对成肌细胞的生理作用知之甚少。在这项研究中，我们将 α-MG 应用于使用 2% HS 诱导分化的 C2C12 细胞，并分析了 α-MS 在成肌分化过程中的生理作用及其潜在机制。α-MG 以浓度依赖的方式增加 C2C12 细胞的存活率。对细胞形态变化的分析表明，α-MG 显著增强了 C2C12 成肌细胞的成肌分化，而线粒体数量仅受到轻微影响。对 C2C12 细胞中分化相关蛋白的表达分析表明，α-MG 促进了 MyoD 和 Myogenin 的表达。因此，本研究揭示了 α-MG 可改善 C2C12 成肌细胞的存活和成肌分化。
