Kutlu Hüseyin Haydar, Avcı Meltem, Dal Tuba, Arı Oğuz, Durmaz Rıza
Medical Microbiology Department, Faculty of Medicine, Usak University.
Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Usak University.
Jpn J Infect Dis. 2020 May 29. doi: 10.7883/yoken.JJJID.2019.536.
Myroides spp. are low-grade opportunistic pathogens. There were only a few outbreaks due to Myroides spp. described in the literature to date. We report a healthcare-associated outbreak of urinary tract infections caused by Myroides odoratimimus in a Turkish hospital. From March to May 2019, six strains of M. odoratimimus were isolated from the urine samples of patients hospitalized in the intensive care units (ICUs). After identification and antibiotic susceptibility testing with VITEK 2 system, MALDI-TOF-MS and 16S rRNA based sequencing methods were performed for confirmation and species level identification. Pulsed-field gel electrophoresis (PFGE) was used to investigate clonal relatedness of the isolates. All the patients were immunocompromised and underwent urinary catheterization. None of them had urinary neoplasm, surgery or calculi. VITEK 2 and MALDI-TOF-MS systems revealed that the isolates belong to the Myroides genus but lacked to identify the isolates at the species level. 16S rRNA based sequencing successfully identified all the isolates as M. odoratimimus. The isolates were resistant to all antibiotics tested. All isolates had indistinguishable PFGE pattern indicating cross-transmission between cases. Although M. odoratimimus is rarely isolated from human specimens, clinicians should be aware of its ability to cause UTIs and outbreaks.
食酸代尔夫特菌属是低级别机会致病菌。迄今为止，文献中仅描述了少数几起因食酸代尔夫特菌属引起的暴发事件。我们报告了土耳其一家医院发生的一起由气味代尔夫特菌引起的医疗保健相关尿路感染暴发。2019年3月至5月，从重症监护病房（ICU）住院患者的尿液样本中分离出6株气味代尔夫特菌。使用VITEK 2系统进行鉴定和抗生素敏感性测试后，采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）和基于16S rRNA的测序方法进行确认和菌种水平鉴定。脉冲场凝胶电泳（PFGE）用于研究分离株的克隆相关性。所有患者均免疫功能低下且接受了导尿术。他们均无泌尿系统肿瘤、手术史或结石。VITEK 2系统和MALDI-TOF-MS系统显示分离株属于代尔夫特菌属，但无法在菌种水平鉴定这些分离株。基于16S rRNA的测序成功将所有分离株鉴定为气味代尔夫特菌。分离株对所有测试抗生素均耐药。所有分离株的PFGE图谱无法区分，表明病例之间存在交叉传播。尽管气味代尔夫特菌很少从人体标本中分离出来，但临床医生应意识到其引起尿路感染和暴发的能力。
