Sriprom Wilasinee, Choodum Aree, Phawachalotorn Chanadda, Wongniramaikul Worawit, Limsakul Wadcharawadee
Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University.
Department of Applied Science and Business Management, King Mongkut's Institute of Technology Ladkrabang, Prince of Chumphon Campus.
Anal Sci. 2020 Oct 10;36(10):1261-1267. doi: 10.2116/analsci.20P117. Epub 2020 May 29.
A tetramethylammonium hydroxide (TMAH)-doped starch film was developed for trinitrotoluene (TNT) detection. A purple Janowsky anion was obtained from the reaction of TNT with released TMAH. When the film was used in conjunction with digital image colorimetry (DIC), rapid quantitative analysis of TNT was achieved. The Red-Green-Blue (RGB) intensities analyzed from digital photographs of the purple product were used to establish calibration curves for TNT. A wide linear range (2.5 to 50 mgL) with good linearity (R > 0.99) was achieved for the quantification of TNT. Good precision (1.73 to 3.74%RSD) was obtained for inter-day tests (n = 5). The films were applied to test four post-blast soil samples and two positive results were observed. The concentrations quantified by DIC were in good agreement with spectrophotometry. The film was able to be stored in a freezer for 3 months with <4.3% change in performance.
制备了一种用于检测三硝基甲苯（TNT）的氢氧化四甲铵（TMAH）掺杂淀粉薄膜。TNT与释放出的TMAH反应生成紫色的雅诺夫斯基阴离子。当该薄膜与数字图像比色法（DIC）结合使用时，可实现对TNT的快速定量分析。从紫色产物的数码照片中分析得到的红-绿-蓝（RGB）强度用于建立TNT的校准曲线。TNT定量分析的线性范围宽（2.5至50 mg/L），线性良好（R>0.99）。日间测试（n = 5）的精密度良好（相对标准偏差为1.73至3.74%）。将该薄膜应用于四个爆炸后土壤样品的检测，观察到两个阳性结果。DIC定量的浓度与分光光度法结果高度一致。该薄膜能够在冰箱中储存3个月，性能变化<4.3%。
