Hui Yi, Yan Shu-Guang, Wang Qian, Li Jing-Tao, Wei Hai-Liang, Shan Yu-Peng
Shanxi University of Chinese Medicine, Xianyang 712046.
The Hospital Afiliated to Shanxi University of Chinese Medicine, Xianyang 712000.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2020 Jan 28;36(1):90-93. doi: 10.12047/j.cjap.5889.2020.020.
To observe the regulatory effect of 6-Shogaol on Notch signal pathway in colonic epithelial cells of mice with ulcerative colitis.
Forty Kunming mice were randomly divided into normal group (n=10) and model group (n=30). The model of ulcerative colitis was induced by free drinking of 2% dextran sulfate sodium salt(DSS). After 15 days, the mice were divided into model group, 6-gingerenol group and positive control group with 10 mice in each group. Normal group and model group were treated with normal saline, 6-gingerenol group was treated with 6-Shogaol 100 mg/(kg·d), positive control group was treated with sulfasalazine 100 mg/(kg·d), for 20 days. The histopathological changes of colon were observed, and the expressions of Hes-1 and Math-1protein in colonic epithelial cells were detected by immunofluorescence double labeling method. The expressions of Notch-1, Hes-1 and Math-1 mRNA in colonic epithelial tissue were detected by RT-PCR. The expressions of Notch-1, Hes-1 and Math-1 protein in colonic epithelial tissue was detected by Western blot.
Compared with the normal group, the expression of Notch-1 and Hes-1 protein and the relative expression of mRNA in colonic epithelium of model group were significantly increased (P＜0.01), while the relative expressions of Math-1 mRNA and protein were decreased significantly (P＜0.01). Compared with the model group, the expressions of Notch-1 and Hes-1 protein and the relative expression of mRNA in colonic epithelium of 6-Shogaol group and sulfasalazine group were decreased significantly(P＜0.01), while the relative expressions of Math-1 mRNA and protein were increased significantly(P＜0.01).
6-Shogaol can inhibit the over activation of Notch pathway and regulate the balance of differentiation between colonic epithelialabsorptive cell line and secretory cell line and repair damaged mucosal tissue.
观察6-姜辣素对溃疡性结肠炎小鼠结肠上皮细胞Notch信号通路的调控作用。
将40只昆明小鼠随机分为正常组（n = 10）和模型组（n = 30）。通过自由饮用2%葡聚糖硫酸钠（DSS）诱导溃疡性结肠炎模型。15天后，将小鼠分为模型组、6-姜酚组和阳性对照组，每组10只。正常组和模型组给予生理盐水灌胃，6-姜酚组给予6-姜辣素100 mg/(kg·d)灌胃，阳性对照组给予柳氮磺胺吡啶100 mg/(kg·d)灌胃，连续20天。观察结肠组织病理学变化，采用免疫荧光双标法检测结肠上皮细胞中Hes-1和Math-1蛋白的表达。采用RT-PCR法检测结肠上皮组织中Notch-1、Hes-1和Math-1 mRNA的表达。采用Western blot法检测结肠上皮组织中Notch-1、Hes-1和Math-1蛋白的表达。
与正常组比较，模型组结肠上皮中Notch-1和Hes-1蛋白表达及mRNA相对表达量显著升高（P＜0.01），而Math-1 mRNA和蛋白相对表达量显著降低（P＜0.01）。与模型组比较，6-姜辣素组和柳氮磺胺吡啶组结肠上皮中Notch-1和Hes-1蛋白表达及mRNA相对表达量显著降低（P＜0.01），而Math-1 mRNA和蛋白相对表达量显著升高（P＜0.01）。
6-姜辣素可抑制Notch通路的过度激活，调节结肠上皮吸收细胞系与分泌细胞系分化平衡，修复受损黏膜组织。
