Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, College of Food & Pharmaceutical Sciences, Ningbo University, Ningbo, Zhejiang, PR China.
National R&D Center for Freshwater Fish Processing, Jiangxi Normal University, Nanchang, Jiangxi 330022, PR China.
Future Microbiol. 2020 May;15:485-496. doi: 10.2217/fmb-2019-0219. Epub 2020 Jun 1.
Confirmation of the enzymatic activity of Class A sortase (SrtA) in probiotic strain associated with the adhesion properties. SrtA from ATCC 4356 was purified and its enzymatic properties was investigated by site-directed mutagenesis approach and the sensitivity to metal ions was also detected. SrtA of ATCC4356 can recognize LPxTG and LPxTD sorting motifs. The active sites of SrtA include His137, Cys198 and Arg205. Furthermore, acacetin can increase the activity of SrtA, while phenyl vinyl sulfone could effectively inhibit the activity of SrtA with an IC of 143.32 μg/ml. The adhesion ability of was also decreased resulting from the inhibition of SrtA activity. The unique properties of SrtA of can provide some insights into the development of high-adhesion strains in the GI tract.
确认与粘附特性相关的益生菌菌株中 A 类 sortase（SrtA）的酶活性。从 ATCC 4356 中纯化 SrtA，并通过定点突变方法研究其酶特性，还检测了其对金属离子的敏感性。ATCC4356 的 SrtA 可以识别 LPxTG 和 LPxTD 分拣基序。SrtA 的活性位点包括 His137、Cys198 和 Arg205。此外，白杨素可以增加 SrtA 的活性，而苯乙烯基砜可以有效抑制 SrtA 的活性，IC 为 143.32μg/ml。SrtA 活性的抑制也降低了 的粘附能力。可以为胃肠道中高粘附性菌株的开发提供一些见解。
