猫血浆促肾上腺皮质激素：化学发光测定法的验证及库欣氏病、原发性肾上腺皮质功能减退症和其他疾病猫中的浓度。
Feline plasma adrenocorticotropic hormone: validation of a chemiluminescent assay and concentrations in cats with hypercortisolism, primary hypoadrenocorticism and other diseases.
机构信息
Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia, Bologna, Italy.
Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
出版信息
J Feline Med Surg. 2021 Feb;23(2):67-73. doi: 10.1177/1098612X20925686. Epub 2020 Jun 1.
OBJECTIVES
The aims of this study were to validate a commercially available chemiluminescent assay for measurement of feline plasma adrenocorticotropic hormone concentration (ACTH), to determine the normal reference interval (RI) of plasma ACTH in healthy cats, to assess plasma ACTH in cats with naturally occurring hypercortisolism (HC), primary hypoadrenocorticism (PH) and other diseases (OD), and to evaluate the effect of aprotinin on plasma ACTH degradation.
METHODS
Forty healthy cats, 10 with HC, 11 with PH and 30 with OD, were included. The chemiluminescent enzyme immunometric assay was evaluated by measurement of intra-assay precision, interassay precision and linearity. The RI for plasma ACTH in healthy cats was established using robust methods. Plasma ACTH of samples collected with and without aprotinin, stored at 4°C and assayed over a 6-day period, was measured.
RESULTS
The intra-assay coefficients of variance (CVs) ranged from 2.7% to 4.3% and interassay CVs from 3.3% to 10.7%. Dilution studies showed excellent accuracy (R >0.99). The RI for plasma ACTH in healthy cats was 32-370 pg/ml. Plasma ACTH was not significantly different between healthy cats and the OD group. Cats with pituitary-dependent hypercortisolism (PDH) and PH had significantly higher plasma ACTH than the other groups. Plasma ACTH did not show significant differences when samples collected with and without aprotinin were compared.
CONCLUSIONS AND RELEVANCE
The Immulite chemiluminescent assay is a valid technique for measuring plasma ACTH in cats and the RI of plasma ACTH is quite wide. Owing to the low overlap between healthy or OD cats and cats with HC or PH, the measurement of plasma ACTH appears to be useful and should be included in the diagnostic work-up when HC or PH are suspected. Furthermore, the measurement of plasma ACTH may be an accurate test for differentiating PDH from adrenal-dependent hypercortisolism.
目的
本研究旨在验证一种市售的用于测量猫血浆促肾上腺皮质激素浓度（ACTH）的化学发光测定法的有效性，确定健康猫的血浆 ACTH 正常参考区间（RI），评估患有自发性库欣综合征（HC）、原发性肾上腺皮质功能减退症（PH）和其他疾病（OD）的猫的血浆 ACTH，并评估抑肽酶对血浆 ACTH 降解的影响。
方法
纳入 40 只健康猫、10 只 HC 猫、11 只 PH 猫和 30 只 OD 猫。通过测量批内精密度、批间精密度和线性来评估化学发光酶免疫测定法。使用稳健的方法确定健康猫的血浆 ACTH RI。测量了使用和不使用抑肽酶采集的、在 4°C 下储存并在 6 天内检测的样本的血浆 ACTH。
结果
批内变异系数（CVs）范围为 2.7%至 4.3%，批间 CVs 范围为 3.3%至 10.7%。稀释研究显示出极好的准确性（R>0.99）。健康猫的血浆 ACTH RI 为 32-370pg/ml。健康猫和 OD 组之间的血浆 ACTH 无显著差异。垂体依赖性库欣综合征（PDH）和 PH 猫的血浆 ACTH 明显高于其他组。比较使用和不使用抑肽酶采集的样本时，血浆 ACTH 无显著差异。
结论和相关性
Immulite 化学发光测定法是一种用于测量猫血浆 ACTH 的有效技术，血浆 ACTH 的 RI 非常宽。由于健康或 OD 猫与 HC 或 PH 猫之间的重叠率较低，因此测量血浆 ACTH 似乎有用，并且在怀疑 HC 或 PH 时应将其纳入诊断性检查。此外，测量血浆 ACTH 可能是区分 PDH 与肾上腺依赖性库欣综合征的准确检查。
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