Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China; College of Chemistry and Chemical Engineering, Taishan University, Taian, 271021, China.
Biosens Bioelectron. 2020 Sep 15;164:112317. doi: 10.1016/j.bios.2020.112317. Epub 2020 May 22.
An antifouling electrochemical biosensing platform was constructed based on conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) planted with designed peptides. The designed peptides containing doping and antifouling sequences were anchored to an electrode surface, followed by the electrochemical polymerization of PEDOT. The negatively charged doping sequence of the peptide was gradually doped into the PEDOT during the polymerization process, and by controlling the polymerization time, it was able to exactly dope the whole doping sequence into the PEDOT film, leaving the antifouling sequence of the peptide stretched out of the PEDOT surface. Therefore, an excellent conducting and antifouling platform was constructed just like planting a peptide tree in the PEDOT soil. With antibodies immobilized on the peptide, an antifouling electrochemical biosensor for the detection of a typical biomarker CA15-3 was developed. Owing to the unique properties of the conducting polymer PEDOT and the antifouling peptide, the electrochemical biosensor exhibited high sensitivity and long-term stability, and it was capable of detecting CA15-3 in serum of breast cancer patients without suffering from biofouling. The strategy of planting designed antifouling peptides in conducting polymers offered an effective way to develop electrochemical sensors for practical biomarkers assaying in complex biological samples.
基于种植设计肽的导电聚合物聚（3,4-亚乙基二氧噻吩）（PEDOT）构建了一种抗污电化学生物传感平台。含有掺杂和抗污序列的设计肽被锚定到电极表面，然后进行 PEDOT 的电化学聚合。肽的带负电荷的掺杂序列在聚合过程中逐渐掺杂到 PEDOT 中，通过控制聚合时间，能够将整个掺杂序列精确地掺杂到 PEDOT 薄膜中，使肽的抗污序列从 PEDOT 表面伸出。因此，就像在 PEDOT 土壤中种植肽树一样，构建了一个出色的导电和抗污平台。通过将抗体固定在肽上，开发了用于检测典型生物标志物 CA15-3 的抗污电化学生物传感器。由于导电聚合物 PEDOT 和抗污肽的独特性质，电化学生物传感器表现出高灵敏度和长期稳定性，并且能够在没有生物污垢的情况下检测乳腺癌患者血清中的 CA15-3。在导电聚合物中种植设计的抗污肽的策略为开发用于实际生物标志物分析的复杂生物样品中的电化学传感器提供了一种有效方法。
