Paris-Lodron-University of Salzburg, Department of Biosciences, Hellbrunner Str. 34, 5020 Salzburg, Austria.
Nucleic Acids Res. 2020 Jul 2;48(W1):W31-W35. doi: 10.1093/nar/gkaa366.
Frequently, the complete functional units of biological molecules are assemblies of protein and nucleic acid chains. Stunning examples are the complex structures of ribosomes. Here, we present TopMatch-web, a computational tool for the study of the three-dimensional structure, function and evolution of such molecules. The unique feature of TopMatch is its ability to match the protein as well as nucleic acid chains of complete molecular assemblies simultaneously. The resulting structural alignments are visualized instantly using the high-performance molecular viewer NGL. We use the mitochondrial ribosomes of human and yeast as an example to demonstrate the capabilities of TopMatch-web. The service responds immediately, enabling the interactive study of many pairwise alignments of large molecular assemblies in a single session. TopMatch-web is freely accessible at https://topmatch.services.came.sbg.ac.at.
通常，生物分子的完整功能单元是蛋白质和核酸链的组装体。核糖体的复杂结构就是令人惊叹的例子。在这里，我们介绍 TopMatch-web，这是一种用于研究此类分子的三维结构、功能和进化的计算工具。TopMatch 的独特之处在于它能够同时匹配完整分子组装体的蛋白质和核酸链。使用高性能的分子查看器 NGL 可以立即可视化得到的结构比对。我们用人和酵母的线粒体核糖体作为示例来演示 TopMatch-web 的功能。该服务响应迅速，允许在单个会话中交互式地研究大型分子组装体的许多两两比对。TopMatch-web 可在 https://topmatch.services.came.sbg.ac.at 免费访问。
