Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Instituto Nacional de Salud, Lima, Peru.
Toxicon. 2020 Sep;184:19-27. doi: 10.1016/j.toxicon.2020.05.016. Epub 2020 May 29.
Bothrops brazili is a pitviper from Amazonian region, responsible for many accidents in Peru. Despite its relevance, its venom has not been extensively characterized. In the present work, Bothrops brazili venom (BbV) components were analyzed by RP-HPLC, SDS-PAGE and MALDI-TOF/TOF. Approximately 37 proteins were identified, belonging to 7 families. Snake venom metalloproteinases (SVMPs) were the most abundant proteins of the venom (33.05%), followed by snake venom serine proteinases (SVSPs, 26.11%), phospholipases A (PLA, 25.57%), snake C-type lectins (CTLs, 9.61%), L-aminoacid oxidase (LAAO, 3.80%), cystein-rich secretory proteins (CRISP, 1.67%) and Bradykinin-potentiating peptide (BPP, 0.20%). In vitro enzymatic activities of BbV showed high levels of SVMP activity and reduced Hyal activity in comparison with other bothropic venoms. Furthermore, BbV reduced VERO cells viability. ELISA and Western Blotting showed that both Peruvian and Brazilian bothropic antivenoms were able to recognize BbV components. This work provides an overview of BbV venom content and indicates a potential efficiency of Peruvian and Brazilian antivenoms to treat accidents with this species.
巴西矛头蝮是一种来自亚马逊地区的矛头蝮蛇，在秘鲁造成了许多事故。尽管它很重要，但它的毒液还没有得到广泛的描述。在本工作中，我们通过 RP-HPLC、SDS-PAGE 和 MALDI-TOF/TOF 分析了巴西矛头蝮蛇毒液（BbV）的成分。鉴定出约 37 种蛋白质，属于 7 个家族。蛇毒金属蛋白酶（SVMPs）是毒液中最丰富的蛋白质（33.05%），其次是蛇毒丝氨酸蛋白酶（SVSPs，26.11%）、磷脂酶 A（PLA，25.57%）、蛇 C 型凝集素（CTLs，9.61%）、L-氨基酸氧化酶（LAAO，3.80%）、富含半胱氨酸的分泌蛋白（CRISP，1.67%）和缓激肽增效肽（BPP，0.20%）。BbV 的体外酶活性显示出高水平的 SVMP 活性和与其他矛头蝮蛇毒液相比降低的 Hyal 活性。此外，BbV 降低了 VERO 细胞的活力。ELISA 和 Western Blotting 表明，秘鲁和巴西的抗蛇毒血清都能够识别 BbV 的成分。这项工作提供了 BbV 毒液成分的概述，并表明秘鲁和巴西抗蛇毒血清对治疗该物种引起的事故具有潜在的效果。
