Aymen Souid, Morena Gabriele, Vincenzo Longo, Laura Pucci, Lorenza Bellani, Abderrazak Smaoui, Chedly Abdelly, Karim Ben Hamed
Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cedria, BP 901, Hammam Lif 2050, Tunisia.
National Research Council, Institute of Biology and Agricultural Biotechnology (IBBA), Pisa Unit, Research Area of Pisa, Via Moruzzi 1, 56124 Pisa, Italy.
Funct Plant Biol. 2016 Jul;43(7):607-619. doi: 10.1071/FP15284.
In this work we studied the effect of salinity (ranging from 50 to 500mM NaCl) on the physiological and the antioxidant responses of the local halophyte Limonium delicatulum Kuntze. We based our analysis on 12 biochemical assays that are commonly used to measure the antioxidant responses under stress such as oxidative stress markers, enzymes activities and polyphenolic compounds. Our aim was to study parameters that are strongly correlated with the growth response to salinity. Results showed two different growth responses depending on the concentration of NaCl in the medium. Under 50 to 200mM, the growth was stimulated before it decreased significantly at 300-500mM. L. delicatulum revealed a good aptitude to maintain photosynthetic machinery by increasing the concentrations of photosynthetic pigments, which is essential for the stabilisation of photosystems and the photosynthesis process under optimal NaCl concentration. Their breakdown at higher salinity decreased the photosynthetic performance of plants resulting in growth inhibition. Moreover, to reduce the damaging effect of oxidative stress and to tolerate the accumulation of salt ions, L. delicatulum induced the activities of their antioxidant enzymes more than their contents in polyphenolic compounds.
在这项工作中，我们研究了盐度（50至500mM NaCl）对当地盐生植物细叶补血草（Limonium delicatulum Kuntze）生理和抗氧化反应的影响。我们的分析基于12种生化检测方法，这些方法通常用于测量胁迫下的抗氧化反应，如氧化应激标志物、酶活性和多酚类化合物。我们的目的是研究与盐度生长反应密切相关的参数。结果表明，根据培养基中NaCl的浓度，生长反应分为两种不同类型。在50至200mM时，生长受到刺激，而在300 - 500mM时显著下降。细叶补血草表现出良好的能力，通过增加光合色素浓度来维持光合机制，这对于在最佳NaCl浓度下稳定光系统和光合作用过程至关重要。在较高盐度下它们的分解降低了植物的光合性能，导致生长受到抑制。此外，为了降低氧化应激的破坏作用并耐受盐离子的积累，细叶补血草诱导其抗氧化酶的活性，而不是多酚类化合物的含量。
