Wang Jian-Jun, Lin Wei-Hu, Zhao Yan-Ting, Meng Cheng, Ma An-Wei, Xue Long-Hai, Kuang Yu, Tian Pei
State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu Province, 730000, China.
School of Life Sciences, Lanzhou University, Lanzhou, Gansu Province, 730000, China.
Funct Plant Biol. 2017 Oct;44(10):1007-1015. doi: 10.1071/FP16410.
The interaction effects between temperature and soil moisture on Festuca sinensis Keng ex E.B.Alexeev were analysed to determine how F. sinensis responds to these environmental conditions. A pot experiment was conducted in a greenhouse under simulated growth conditions with four soil moisture contents (80, 65, 50 and 35% relative saturation moisture content) and three temperature conditions (15, 20 and 25°C). Physiological (relative water content and root activity) and biochemical parameters (chlorophyll, peroxidase (POD), malondialdehyde (MDA), soluble protein, soluble sugar and free proline) were evaluated at the seedling stage. Results showed that with a decrease in soil water content, the POD activities, MDA content, soluble protein content, soluble sugar content and free proline content of plants under the 15°C and 20°C treatments initially decreased and then increased, whereas they increased with a decrease of soil water content at 25°C. The relative water contents of plants under the three temperature treatments decreased with a decreasing soil moisture content, but then increased temperature significantly reduced the relative water content of the seedlings under low soil water content. The chlorophyll contents of plants under the 25°C treatment decreased with a decrease of soil moisture content, but those of plants under the 15°C and 20°C treatments initially increased and then decreased. The root activities of plants under the 15°C and 20°C treatments increased with a decreasing soil moisture content; however, those of plants under the 25°C treatment initially increased and then decreased. Thus, results indicated that changes of temperature and soil moisture content had significant and complicated effects on the physiological-biochemical characteristics of F. sinensis; the conditions of 20°C and 65% RSMC had positive effects on F. sinensis seedling growth and the appropriate drought stress could promote the growth of seedling roots under the three different temperature conditions. In conclusion, F. sinensis seedlings could adapt to certain changes in the ecological environment by regulating their physiological and biochemical reactions.
分析了温度与土壤湿度对中华羊茅（Festuca sinensis Keng ex E.B.Alexeev）的交互作用，以确定中华羊茅对这些环境条件的响应方式。在温室中进行盆栽试验，模拟生长条件设置了4种土壤湿度（相对饱和含水量分别为80%、65%、50%和35%）和3种温度条件（15℃、20℃和25℃）。在幼苗期评估了生理参数（相对含水量和根系活力）和生化参数（叶绿素、过氧化物酶（POD）、丙二醛（MDA）、可溶性蛋白、可溶性糖和游离脯氨酸）。结果表明，随着土壤含水量降低，15℃和20℃处理下植株的POD活性、MDA含量、可溶性蛋白含量、可溶性糖含量和游离脯氨酸含量先降低后升高，而在25℃时则随土壤含水量降低而升高。三种温度处理下植株的相对含水量均随土壤湿度降低而降低，但温度升高显著降低了低土壤含水量下幼苗的相对含水量。25℃处理下植株的叶绿素含量随土壤湿度降低而降低，而15℃和20℃处理下植株的叶绿素含量先升高后降低。15℃和20℃处理下植株的根系活力随土壤含水量降低而升高；然而，25℃处理下植株的根系活力先升高后降低。因此，结果表明温度和土壤含水量的变化对中华羊茅的生理生化特性具有显著且复杂的影响；20℃和65%相对饱和含水量条件对中华羊茅幼苗生长有积极影响，在三种不同温度条件下适当的干旱胁迫可促进幼苗根系生长。总之，中华羊茅幼苗可通过调节其生理生化反应来适应生态环境的某些变化。
