Malomvölgyi B, Hadházy P, Magyar K
Department of Pharmacodynamics, Semmelweis Medical School, Budapest, Hungary.
Biomed Biochim Acta. 1988;47(10-11):S125-8.
Helically cut femoral artery strips from humans (bypass surgery), dogs and rabbit were set up for isometric recording. The endothelial layer was removed by rubbing, hence the vascular strips used in this study are considered to be arterial smooth muscle preparations without endothelium. The indomethacin-, prostaglandin F2 alpha- and PGI2-induced changes in tone were determined. Indomethacin (3/mumol/l) potentiated the contractile responses of human and canine vessels to PGF2 alpha by 60.0 +/- 10.4 and 108 +/- 12%, respectively. By contrast, the PGF2 alpha-induced contractions of rabbit arteries were not enhanced by the cyclooxygenase inhibitor. PGI2 diminished the tone of the PGF2 alpha-contracted vessels obtained from all the three species in a concentration-related manner. The potency of the relaxant prostanoid differed, however, markedly (p less than 0.02) from species to species. The IC50 values (molar concentrations of PGI2 producing 50 percent reduction in the PGF2 alpha-induced tone) were 20.8 +/- 1.9, 133 +/- 24 and 286 +/- 52 nmol/l for human, dog and rabbit arteries, respectively. The results clearly demonstrate a significant interspecies difference in the prostacyclin-sensitivity of the blood vessels studied. The differential responsiveness to indomethacin may reflect species-difference in the regulatory function of PGI2 in arterial smooth muscle tone.
将取自人类（搭桥手术）、犬类和兔类的股动脉条进行螺旋切割，用于等长记录。通过摩擦去除内皮，因此本研究中使用的血管条被视为无内皮的动脉平滑肌制剂。测定了吲哚美辛、前列腺素F2α和前列环素引起的张力变化。吲哚美辛（3 μmol/l）分别使人类和犬类血管对前列腺素F2α的收缩反应增强了60.0±10.4%和108±12%。相比之下，环氧化酶抑制剂并未增强兔动脉由前列腺素F2α诱导的收缩。前列环素以浓度相关的方式降低了从所有三个物种获得的由前列腺素F2α收缩的血管的张力。然而，不同物种间，舒张性前列腺素的效力存在显著差异（p<0.02）。人类、犬类和兔类动脉的IC50值（使前列腺素F2α诱导的张力降低50%的前列环素的摩尔浓度）分别为20.8±1.9、133±24和286±52 nmol/l。结果清楚地表明，在所研究的血管中，前列环素敏感性存在显著的种间差异。对吲哚美辛的不同反应性可能反映了前列环素在动脉平滑肌张力调节功能上的种间差异。
