Shimizu H, Morimoto K, Yoshimine T, Shimada N, Masana Y, Hayakawa T, Hashimoto T
Department of Neurosurgery, Osaka University Medical School, Japan.
No To Shinkei. 1988 Dec;40(12):1163-8.
Ketamine-anesthetized gerbils were implanted with 0.5 mm diameter microdialysis tubing through the cortex, hippocampus and thalamus with a stereotaxic instrument. The cannula was perfused with artificial cerebrospinal fluid at a flow rate of 2 microliters/min. Samples of the perfusate representing the cerebral interstitial fluid were collected and subsequently analyzed for their content of S-adenosyl-L-methionine (SAM) with high performance liquid chromatography (HPLC). In vitro tests using the dialysis cannula gave SAM recovery which varied from 3.44 +/- 0.31% (n = 3) at a perfusion flow rate of 8 microliters/min to 43.8 +/- 3.81% (n = 3) at 0.5 microliters/min (mean +/- SEM). The in vivo SAM content of the perfusate was studied and the endogenous SAM level in cerebral interstitial fluid of gerbil was 3.49 +/- 1.52 nmol per ml (n = 3). Intraperitoneal injection of SAM (250 mg/kg) produced 10-fold increases in the perfusate SAM concentration. After the experiment the brains were checked by immunopathological study using anti-albumin antibody to examine the degree of damage to the blood-brain barrier permeability. These results show that intracerebral microdialysis offers several potential advantages for studies of extracellular neurochemistry.
使用立体定位仪，将直径为0.5毫米的微透析管通过皮质、海马体和丘脑植入氯胺酮麻醉的沙鼠体内。以2微升/分钟的流速向插管中灌注人工脑脊液。收集代表脑间质液的灌注液样本，随后用高效液相色谱法（HPLC）分析其S-腺苷-L-甲硫氨酸（SAM）含量。使用透析插管进行的体外试验表明，SAM回收率在灌注流速为8微升/分钟时为3.44±0.31%（n = 3），在0.5微升/分钟时为43.8±3.81%（n = 3）（平均值±标准误）。研究了灌注液中的体内SAM含量，沙鼠脑间质液中的内源性SAM水平为每毫升3.49±1.52纳摩尔（n = 3）。腹腔注射SAM（250毫克/千克）使灌注液中SAM浓度增加了10倍。实验结束后，使用抗白蛋白抗体通过免疫病理学研究检查大脑，以检测血脑屏障通透性的损伤程度。这些结果表明，脑内微透析在细胞外神经化学研究中具有几个潜在的优势。
