Niu S, Zhao Z G, Lyu X M, Zhao M, Wang X Z, Liu W N, Zhao W, Zhang X H, Wang Y
Second Department of Endocrinology, Shijiazhuang First Hospital, Shijiazhuang 050011, China.
Department of Pathology, Shijiazhuang First Hospital, Shijiazhuang 050011, China.
Zhonghua Zhong Liu Za Zhi. 2020 May 23;42(5):391-395. doi: 10.3760/cma.j.cn112152-112152-20190906-00580.
To investigate the expression of IGF1R-Ras and RAGE-HMGB1 signaling pathways in colorectal cancer patients with type 2 diabetes mellitus and their significance. The resected cancer tissues were obtained from 59 patients with colorectal cancer (CRC), including 29 patients with type 2 diabetes mellitus (CRC/DM group) and 30 with CRC alone (CRC group). The expressions of IGF1R, Ras, RAGE and HMGB1 in cancer tissues were detected by immunohistochemistry. The differences between the two groups were compared and the relationship between the expression and clinicopathological characteristics was analyzed. In CRC/DM group, the positive rates of IGF1R and Ras were both 65.5% (19/29), and 51.7% (15/29) patients had IGF1R+ Ras+ immunophenotype, which were significantly higher than those in CRC group [33.3% (10/30), 36.7% (11/30) and 20.0% (6/30); =0.013, 0.027 and 0.011, respectively]. The expression of IGF1R and Ras in CRC / DM group was positively correlated (=0.479, =0.017). The positive rate of RAGE expression in CRC group and CRC/DM group was 70.0% (21/30) and 72.4% (21/29) respectively, and the positive rate of HMGB1 expression was 46.7% (14/30) and 58.6% (17/29) respectively, neither was observed with significant difference (=0.358 and 0.838). However, the proportion of patients with RAGE+ HMGB1+ immunophenotype in CRC/DM group [55.2% (16/29)] was higher than that in CRC Group [26.7% (8/30)] which was statistically significant (=0.026), and the expression of both proteins was positively correlated in CRC/DM group (=0.578, =0.003). The clinicopathological analysis showed that in both groups the expression of IGF1R, Ras, RAGE and HMGB1 had no correlation with the sex, age, differentiation degree, tumor length, T stage and lymph node metastasis (>0.05). Both IGF1R-Ras and RAGE-HMGB1 pathways may be involved in the oncogenesis of colorectal cancer in patients with type 2 diabetes.
探讨2型糖尿病结直肠癌患者中IGF1R-Ras和RAGE-HMGB1信号通路的表达及其意义。收集59例结直肠癌患者的癌组织，其中29例合并2型糖尿病（结直肠癌/糖尿病组），30例单纯结直肠癌患者（结直肠癌组）。采用免疫组织化学法检测癌组织中IGF1R、Ras、RAGE和HMGB1的表达。比较两组间差异，并分析其表达与临床病理特征的关系。结直肠癌/糖尿病组中，IGF1R和Ras的阳性率均为65.5%（19/29），51.7%（15/29）的患者具有IGF1R+Ras+免疫表型，均显著高于结直肠癌组[33.3%（10/30）、36.7%（11/30）和20.0%（6/30）；P分别为0.013、0.027和0.011]。结直肠癌/糖尿病组中IGF1R和Ras的表达呈正相关（r=0.479，P=0.017）。结直肠癌组和结直肠癌/糖尿病组中RAGE表达的阳性率分别为70.0%（21/30）和72.4%（21/29），HMGB1表达的阳性率分别为46.7%（14/30）和58.6%（17/29），均无显著差异（P=0.358和0.838）。然而，结直肠癌/糖尿病组中RAGE+HMGB1+免疫表型的患者比例[55.2%（16/29）]高于结直肠癌组[26.7%（8/30）]，差异有统计学意义（P=0.026），且在结直肠癌/糖尿病组中两种蛋白的表达呈正相关（r=0.578，P=0.003）。临床病理分析显示，两组中IGF1R、Ras、RAGE和HMGB1的表达与性别、年龄、分化程度、肿瘤长度、T分期及淋巴结转移均无相关性（P>0.05）。IGF1R-Ras和RAGE-HMGB1信号通路可能均参与2型糖尿病患者结直肠癌的发生发展。
