Université Clermont Auvergne, INRAE, UNH, CRNH Auvergne, F-63000, Clermont-Ferrand, France; Department of Internal Medicine, Division of Cardiovascular Medicine, School of Medicine, University of California Davis, Davis, CA 95616, United States.
Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wroclaw, Poland.
Nutr Metab Cardiovasc Dis. 2020 Jun 25;30(7):1201-1215. doi: 10.1016/j.numecd.2020.04.007. Epub 2020 Apr 20.
We previously showed that 12-month high-fat diet (HFD) in pigs led to fattening and increased artery intima-media-thickness, which were partly reversed after 3-month return to control diet (CD). The aim of this study was to decipher underlying mechanism of action by using transcriptomic analyses of intima and media of aorta.
Thirty-two pigs were divided into three groups: CD for 12 months; HFD for 12 months; switch diet group (regression diet; RD): HFD for 9 months followed by CD for 3 months. After 12 months, RNA was isolated from aorta intima and media for nutrigenomic analyses. HFD significantly affected gene expression in intima, while RD gene expression profile was distinct from the CD group. This suggests that switch to CD is not sufficient to correct gene expression alterations induced by HFD but counteracted expression of a group of genes. HFD also affected gene expression in media and as for intima, the expression profile of media of pigs on RD differed from that of these on CD.
This study revealed nutrigenomic modifications induced by long-term HFD consumption on arterial intima and media. The return to CD was not sufficient to counteract the genomic effect of HFD.
我们之前的研究表明，12 个月的高脂肪饮食（HFD）会导致猪肥胖，并增加动脉内膜-中膜厚度，而在恢复正常饮食（CD）3 个月后，这些变化部分得到逆转。本研究旨在通过对主动脉内膜和中膜的转录组分析，揭示其潜在的作用机制。
32 头猪被分为三组：12 个月的 CD；12 个月的 HFD；切换饮食组（回归饮食；RD）：9 个月的 HFD 后再用 3 个月的 CD。12 个月后，从主动脉内膜和中膜分离 RNA 进行营养基因组分析。HFD 显著影响了内膜的基因表达，而 RD 的基因表达谱与 CD 组不同。这表明切换到 CD 不足以纠正 HFD 引起的基因表达改变，但会拮抗一组基因的表达。HFD 还影响了中膜的基因表达，与内膜一样，RD 组猪的中膜表达谱与 CD 组不同。
本研究揭示了长期 HFD 摄入对动脉内膜和中膜的营养基因组修饰。恢复 CD 饮食不足以对抗 HFD 的基因组效应。
